用离子选择性微电极测量短杆菌肽通道中的流动电位。
Streaming potentials in gramicidin channels measured with ion-selective microelectrodes.
作者信息
Tripathi S, Hladky S B
机构信息
Tata Institute of Fundamental Research, Mumbai, India.
出版信息
Biophys J. 1998 Jun;74(6):2912-7. doi: 10.1016/S0006-3495(98)77998-6.
Abstract
Streaming potentials have been measured for gramicidin channels with a new method employing ion-selective microelectrodes. It is shown that ideally ion-selective electrodes placed at the membrane surface record the true streaming potential. Using this method for ion concentrations below 100 mM, approximately seven water molecules are transported whenever a sodium, potassium, or cesium ion, passes through the channel. This new method confirms earlier measurements (Rosenberg, P.A., and A. Finkelstein. 1978. Interaction of ions and water in gramicidin A channels. J. Gen. Physiol. 72:327-340) in which the streaming potentials were calculated as the difference between electrical potentials measured in the presence of gramicidin and in the presence of the ion carriers valinomycin and nonactin.
摘要
利用一种采用离子选择性微电极的新方法测量了短杆菌肽通道的流动电势。结果表明，置于膜表面的理想离子选择性电极记录的是真实的流动电势。对于低于100 mM的离子浓度，使用该方法时，每当一个钠、钾或铯离子通过通道时，大约有七个水分子被运输。这种新方法证实了早期的测量结果（罗森伯格，P.A.，和A.芬克尔斯坦。1978年。短杆菌肽A通道中离子与水的相互作用。《普通生理学杂志》72:327 - 340），其中流动电势被计算为在存在短杆菌肽以及存在离子载体缬氨霉素和尼日利亚菌素的情况下所测量的电势差。
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