Kern K A, Norton J A
Cancer Res. 1987 Sep 1;47(17):4706-8.
As sarcomas are known to have accelerated glycolysis, we used the radiolabeled glucose analogue 2-deoxy-d-[U-14C]glucose in autoradiographic imaging studies of a methylcholanthrene-induced rat fibrosarcoma placed in an i.m. site, and in models of pulmonary and hepatic metastases. Fifty muCi of 2-deoxy-d-[U-14C]glucose were injected i.p. into groups of rats bearing tumors in these three sites; sacrifice of animals for imaging was carried out 45 min later. Excellent imaging of sarcoma tissue in all three anatomical sites was obtained, with high visual contrast compared to the normal tissue background. Using densitometry of autoradiographs, tumor/tissue ratios were 7.1 for i.m. tumors, 3.8 for pulmonary metastases, and 2.8 for hepatic metastases. Autoradiographic imaging of sarcomas may be obtained based upon avidity of neoplastic tissue for the glucose analogue 2-deoxy-d-[U-14C] glucose. Such imaging is not dependent upon anatomical site and reproducibly images rat sarcomas in muscle, lung, and liver.
由于已知肉瘤具有加速的糖酵解作用，我们在对植入肌肉部位的甲基胆蒽诱导的大鼠纤维肉瘤以及肺和肝转移模型进行放射自显影成像研究时，使用了放射性标记的葡萄糖类似物2-脱氧-D-[U-14C]葡萄糖。向在这三个部位患有肿瘤的大鼠组腹腔注射50微居里的2-脱氧-D-[U-14C]葡萄糖；45分钟后对动物进行处死以进行成像。在所有三个解剖部位均获得了肉瘤组织的出色成像，与正常组织背景相比具有高视觉对比度。使用放射自显影片的光密度测定法，肌肉内肿瘤的肿瘤/组织比为7.1，肺转移为3.8，肝转移为2.8。肉瘤的放射自显影成像可基于肿瘤组织对葡萄糖类似物2-脱氧-D-[U-14C]葡萄糖的摄取能力获得。这种成像不依赖于解剖部位，并且可重复成像肌肉、肺和肝中的大鼠肉瘤。
