Hammond W G, Gabriel A, Paladugu R R, Azumi N, Hill L R, Benfield J R
Cancer Res. 1987 Oct 1;47(19):5202-6.
Previous studies of chemical carcinogenesis in the lung of Syrian golden hamsters have utilized outbred (nonsyngeneic) animals. Using the endobronchial sustained release implant technique, which causes focally originating cancers in outbred hamsters, we studied the course of bronchial carcinogenesis in two varieties of syngeneic Syrian golden hamsters, the LSH and the F1D strains (BIO 15.16 male X BIO 87.20 female). With either 10% benzo(a)pyrene or 10% methylcholanthrene sustained release implants the time course of epithelial transition from normal to neoplastic was the same for F1D hamsters as previously described for outbred hamsters. Using 10% benzo(a)pyrene sustained release implants the incidence of cancers as a function of time was significantly lower (P less than 0.001) in LSH hamsters as compared to outbred and F1D animals. Of 19 tumors transplanted into syngeneic F1D hamsters, 16 have been successfully propagated by serial transplantation. We conclude that (a) F1D hamsters are comparable to outbred animals in the response of their bronchial epithelium to endobronchial benzo(a)pyrene and methylcholanthrene, (b) there are significant differences in susceptibility to bronchial chemical carcinogenesis among hamster strains, thereby giving opportunity to study potential genetic control mechanisms during bronchial carcinogenesis, and (c) F1D hamsters are suitable for studies of lung cancer biology using tumor transplantation methods.
以往对叙利亚金黄地鼠肺部化学致癌作用的研究使用的是远交（非同基因）动物。我们采用支气管内缓释植入技术（该技术可在远交地鼠中引发局灶性起源的癌症），研究了两种同基因叙利亚金黄地鼠品系LSH和F1D品系（BIO 15.16雄性×BIO 87.20雌性）支气管致癌的过程。对于F1D地鼠，使用10%苯并(a)芘或10%甲基胆蒽缓释植入物时，上皮从正常向肿瘤性转变的时间进程与先前描述的远交地鼠相同。使用10%苯并(a)芘缓释植入物时，与远交地鼠和F1D动物相比，LSH地鼠中癌症发生率随时间的变化显著较低（P小于0.001）。在移植到同基因F1D地鼠体内的19个肿瘤中，有16个已通过连续移植成功传代。我们得出结论：(a) F1D地鼠支气管上皮对支气管内苯并(a)芘和甲基胆蒽的反应与远交动物相当；(b) 地鼠品系之间对支气管化学致癌作用的易感性存在显著差异，从而为研究支气管致癌过程中的潜在遗传控制机制提供了机会；(c) F1D地鼠适用于使用肿瘤移植方法进行肺癌生物学研究。
