Zhao Jiuyan, Zhu Lanlan, Zhang Wei
Emergency Department, Guizhou Province, The Affiliated Hospital of Guizhou Medical University, No. 28, Guiyijie Road, Liuguangmen, Guiyang City 550004, China.
Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Nanchang University, Nanchang University, Yong Wai Zheng Jie No. 17, Nanchang 330006, China.
Evid Based Complement Alternat Med. 2022 Sep 30;2022:5665921. doi: 10.1155/2022/5665921. eCollection 2022.
The PVF Tedlar is widely used for gas collection in clinical diagnostics and environmental research. However, sample collection is frequently associated with the degradation, adsorption, or transformation of sensitive chemicals. Here, we explore to what extent the Tedlar bag collection effects the composition of expired breath samples. Collected breath samples were analyzed using the EESI-MS technique after the storage time of 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h, respectively. Our results demonstrated the gradual MS signal decay after 3 h storage. The decay rate of 3 h is about 45% and 6 h is about 88%. Therefore, the Tedlar bag is suggested as a reliable breath holder on the time scale of <3 h.
聚氟乙烯（PVF）泰德拉袋广泛用于临床诊断和环境研究中的气体收集。然而，样品采集常常与敏感化学物质的降解、吸附或转化有关。在此，我们探究泰德拉袋收集对呼出气体样本成分的影响程度。分别在储存30分钟、1小时、2小时、3小时、4小时、5小时和6小时后，使用常压敞开式离子源质谱（EESI-MS）技术对收集的呼出气体样本进行分析。我们的结果表明，储存3小时后质谱信号逐渐衰减。3小时的衰减率约为45%，6小时约为88%。因此，建议在小于3小时的时间范围内，泰德拉袋可作为可靠的气体收集容器。
