Lan Lin, Wang Shengyu, Duan Shuhua, Zhou Xiangyu, Li Yufeng
Molecular Biology Laboratory, College of Food and Biological Engineering, Pidu District, Xihua University, Chengdu City, Sichuan Province, China.
Evid Based Complement Alternat Med. 2022 Sep 30;2022:1259093. doi: 10.1155/2022/1259093. eCollection 2022.
Vision loss is primarily caused by age-related macular degeneration (AMD) due to oxidative retinal pigment epithelial (RPE) cell injury. Carotenoid utilization is deemed a possible strategy for treating AMD. has advantages like immunomodulatory, anti-inflammatory, and antioxidative characteristics. This paper assessed the possible protective influence of carotenoids obtained by isolating and purifying the (CMCT) into human RPE cells (ARPE-19) damaged by hydrogen peroxide (HO). The findings demonstrated that CMCT safeguarded the ARPE-19 cells against the damage and apoptosis caused by HO and oxidative stress via Bcl-2 protein upregulation, as well as the expression of Bax and cleaved caspase-3 protein. In addition, CMCT treatment increased cell survival and restricted the generation of HO-induced reactive oxygen species (ROS) and the protein expression of NADPH oxidase-1 (NOX1). Additionally, the CMCT treatment of HO-induced ARPE-19 cells ameliorated high malondialdehyde (MDA) levels in oxidative stress-induced cells. The catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH) returned to standard levels, which were governed by the higher expression of nuclear Nrf2 protein in the ARPE-19 cells. Moreover, this study showed that CMCT safeguarded the ARPE-19 cells against the damage caused by oxidative stress via its antioxidant activity and antiapoptotic functionality, suggesting the potential therapeutic role of CMCT in AMD prevention and mitigation.
视力丧失主要是由与年龄相关的黄斑变性（AMD）引起的，这是由于视网膜色素上皮（RPE）细胞受到氧化损伤所致。类胡萝卜素的利用被认为是治疗AMD的一种可能策略。 具有免疫调节、抗炎和抗氧化等特性。本文评估了通过分离和纯化 （CMCT） 获得的类胡萝卜素对过氧化氢（HO）损伤的人RPE细胞（ARPE-19）可能产生的保护作用。研究结果表明，CMCT通过上调Bcl-2蛋白以及Bax和裂解的caspase-3蛋白的表达，保护ARPE-19细胞免受HO和氧化应激引起的损伤和凋亡。此外，CMCT处理提高了细胞存活率，限制了HO诱导的活性氧（ROS）的产生以及NADPH氧化酶-1（NOX1）的蛋白表达。此外，CMCT处理HO诱导的ARPE-19细胞改善了氧化应激诱导细胞中高丙二醛（MDA）水平。过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH）恢复到正常水平，这是由ARPE-19细胞中核Nrf2蛋白的高表达所控制的。此外，本研究表明，CMCT通过其抗氧化活性和抗凋亡功能保护ARPE-19细胞免受氧化应激造成的损伤，表明CMCT在AMD预防和缓解方面具有潜在的治疗作用。
