Lignon Julia Somavilla, Pinto Diego Moscarelli, Monteiro Silvia Gonzalez
Veterinarian, MSc., Laboratório de Parasitologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Veterinarian, DSc., Departamento de Veterinária Preventiva, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Braz J Vet Med. 2022 Oct 4;44:e002922. doi: 10.29374/2527-2179.bjvm002922. eCollection 2022.
have medical and veterinary importance, as they play a role as vectors of viruses, protozoa, and nematodes that cause diseases. Despite the relevance to public health, greater attention has been given to other insect vectors. Therefore, the objective of this study was to evaluate the efficiency of the Captor light trap in capturing that could be examined for the presence of DNA. The insects were captured in a rural area of Santa Maria, Rio Grande do Sul, Brazil, where canine and human visceral leishmaniasis have been diagnosed. Adult insects were collected weekly, from 6:00 pm to 6:00 am, for a 12 month period using a Captor brand suction light trap. All were identified at species level. Pools of were tested using the Polymerase Chain Reaction (PCR) technique for the detection of DNA. A total of 16,016 specimens were collected (71.54% females and 28.39% males), divided among seven species. In the DNA analysis, none of the pools showed a positive result for . The data presented demonstrate that the trap is efficient and can be an alternative for use in entomological research. They also demonstrate that, despite the females having hematophagous habits, similar to other vector insects, they did not have contact with in the studied locality.
具有医学和兽医重要性，因为它们作为病毒、原生动物和线虫的传播媒介，会引发疾病。尽管与公共卫生相关，但人们对其他昆虫传播媒介给予了更多关注。因此，本研究的目的是评估Captor诱虫灯诱捕昆虫的效率，这些昆虫可用于检测[某种寄生虫名称]DNA的存在。昆虫在巴西南里奥格兰德州圣玛丽亚的一个农村地区捕获，该地区已诊断出犬类和人类内脏利什曼病。在12个月的时间里，每周从下午6点到早上6点，使用Captor牌吸气式诱虫灯收集成年昆虫。所有昆虫都鉴定到物种水平。将昆虫样本池使用聚合酶链反应（PCR）技术检测[某种寄生虫名称]DNA。总共收集了16,016个标本（71.54%为雌性，28.39%为雄性），分属于七个物种。在DNA分析中，没有一个样本池显示[某种寄生虫名称]呈阳性结果。所呈现的数据表明该诱虫灯是有效的，可作为昆虫学研究的一种替代工具。数据还表明，尽管雌性昆虫具有吸血习性，与其他传播媒介昆虫相似，但在研究地点它们并未接触到[某种寄生虫名称]。
