Silva Elaine A, Andreotti Renato, Dias Edelberto S, Barros Jacqueline C, Brazuna Julia C M
Centro de Controle de Zoonoses, Secretaria Municipal de Saúde, Campo Grande, MS, Brazil.
Exp Parasitol. 2008 Jul;119(3):343-8. doi: 10.1016/j.exppara.2008.03.011. Epub 2008 Mar 30.
Over the past years, leishmaniases have become a public health issue in the Brazilian state of Mato Grosso do Sul, particularly in Campo Grande, the state capital. The purpose of this study was to detect the presence of Leishmania DNA in the population of phlebotomine sandflies using DNA amplification by PCR. Insect captures were carried out from 4 pm. to 7 am for 4 consecutive days each month from October 2005 to September 2006 in 16 neighborhoods located in 7 urban regions of Campo Grande. Traps were placed indoors and in the vicinity of households. As many as 971 males and 203 females were collected. One hundred and five naturally fed females were identified and grouped as 1- to 4-specimen pools. DNA extraction was carried out using whole insects. Lutzomyia longipalpis predominated, accounting for 99.15% of the phlebotomines captured. Also found was Nyssomyia whitmani, the vector of tegumentary leishmaniasis. Abundance was greatest in the vicinity of households (69.8% of the phlebotomines captured). As revealed by PCR, parasites were present in 1.9% of the Leishmania spp. specimens investigated and confirmed for visceral leishmaniasis.
在过去几年中，利什曼病已成为巴西南马托格罗索州的一个公共卫生问题，尤其是在该州首府大坎普。本研究的目的是通过聚合酶链反应（PCR）进行DNA扩增，检测白蛉种群中利什曼原虫DNA的存在情况。2005年10月至2006年9月，每月连续4天，每天从下午4点至上午7点，在大坎普7个城区的16个社区进行昆虫捕捉。诱捕器放置在室内和住户附近。共收集到971只雄虫和203只雌虫。鉴定出105只自然吸血的雌虫，并将其分成1至4个样本池。使用整只昆虫进行DNA提取。长须罗蛉占主导地位，占捕获白蛉的99.15%。还发现了皮肤利什曼病的传播媒介惠特曼按蚊。在住户附近捕获的白蛉数量最多（占捕获白蛉的69.8%）。PCR结果显示，在所调查的利什曼原虫属样本中，1.9%存在寄生虫，并确诊为内脏利什曼病。
