Gravela E, Zuretti M F, Brossa O
Cell Biol Int Rep. 1987 Aug;11(8):573-82. doi: 10.1016/0309-1651(87)90137-8.
We have studied the activity, thiol-dependency and Km of ornithine decarboxylase (ODC) from the following sources: liver of rats subjected to partial hepatectomy or administered thioacetamide, the rat 3924A Morris hepatoma, the rat AH130 Yoshida ascites hepatoma, a mouse transplantable mammary carcinoma and kidney of rats administered testosterone propionate. In order to detect possible changes occurring during in vivo ageing of this enzyme we inhibited protein synthesis with cycloheximide. A gradual decrease of Km during ageing was observed in ODC from liver.
我们研究了来自以下来源的鸟氨酸脱羧酶（ODC）的活性、硫醇依赖性和米氏常数（Km）：接受部分肝切除术或给予硫代乙酰胺的大鼠肝脏、大鼠3924A莫里斯肝癌、大鼠AH130吉田腹水肝癌、一种小鼠可移植乳腺癌以及给予丙酸睾酮的大鼠肾脏。为了检测该酶在体内老化过程中可能发生的变化，我们用放线菌酮抑制蛋白质合成。在肝脏来源的ODC中，观察到老化过程中Km逐渐降低。
