长链非编码RNA HULC对骨肉瘤细胞增殖、迁移和侵袭的影响
Effect of Long Noncoding RNA HULC on Proliferation, Migration, and Invasion of Osteosarcoma Cells.
作者信息
Zhao Ran, Zhou Xin, Zhang Wencan, Li Le
机构信息
Department of Burns and Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, China.
Department of Orthopedic Surgery, Qilu Hospital of Shandong University, Jinan, 250021 Shandong, China.
出版信息
J Oncol. 2022 Sep 30;2022:7526731. doi: 10.1155/2022/7526731. eCollection 2022.
BACKGROUND
Previous studies had shown that lncRNA HULC exhibited different effects in human cancers. However, the role of HULC was not reported in osteosarcoma. Hence, we designed this research to explore the function of HULC in osteosarcoma.
METHODS
Firstly, HULC expression was measured in osteosarcoma tissues and cells via the RT-qPCR assay. The protein expression was detected through western blot. Then, CCK-8 and Transwell assays were conducted to measure cell proliferation, migration, and invasion.
RESULTS
The expression of HULC was obviously higher in osteosarcoma tissues and cells compared with normal control. Moreover, cell proliferation, migration, and invasion were inhibited by HULC knockdown in osteosarcoma cells. HULC overexpression markedly increased osteosarcoma cell proliferation and tumor size in vivo. Furthermore, HULC increased the activity of AKT-PI3K-mTOR pathway by blocking PTEN in osteosarcoma cells. LY294002 inhibited the phosphorylation of AKT, mTOR, and PI3K. Overexpressing HULC enhanced cell migration and invasion of SAOS-2 cells and MG63 cells, while LY294002 reversed the effects.
CONCLUSION
HULC enhanced the progression of osteosarcoma through targeting PTEN.
背景
先前的研究表明，长链非编码RNA HULC在人类癌症中表现出不同的作用。然而，HULC在骨肉瘤中的作用尚未见报道。因此，我们设计了本研究以探讨HULC在骨肉瘤中的功能。
方法
首先，通过RT-qPCR检测骨肉瘤组织和细胞中HULC的表达。通过蛋白质印迹法检测蛋白质表达。然后，进行CCK-8和Transwell实验以测量细胞增殖、迁移和侵袭能力。
结果
与正常对照相比，骨肉瘤组织和细胞中HULC的表达明显更高。此外，骨肉瘤细胞中HULC敲低抑制了细胞增殖、迁移和侵袭。HULC过表达显著增加了体内骨肉瘤细胞的增殖和肿瘤大小。此外，HULC通过在骨肉瘤细胞中阻断PTEN来增加AKT-PI3K-mTOR通路的活性。LY294002抑制了AKT、mTOR和PI3K的磷酸化。过表达HULC增强了SAOS-2细胞和MG63细胞的迁移和侵袭能力，而LY294002逆转了这些作用。
结论
HULC通过靶向PTEN促进骨肉瘤进展。
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