Hergemöller Daniel Fernando, Pelegrine André Antonio, Pasquali Paulo José, Scavone de Macedo Luis Guilherme, Teixeira Marcelo Lucchesi, Moy Peter Karyen, Aloise Antonio Carlos
Department of Implantology, São Leopoldo Mandic School of Dentistry, Campinas, SP, Brazil.
Department of Oral and Maxillofacial Surgery, School of Dentistry, University of California, Los Angeles, CA, USA.
Contemp Clin Dent. 2022 Jul-Sep;13(3):211-216. doi: 10.4103/ccd.ccd_723_20. Epub 2022 Sep 24.
It is known that a large number of mediators involved in osteogenesis can influence bone development and repair; however, whether these mediators could be used as markers of bone maturity has yet to be determined.
To evaluate the expression of osteocalcin (OC) and Runt-related transcription factor 2 (Runx2) in bone biopsies obtained during the reconstruction of atrophic anterior maxillae using particulate bone xenografts with or without association of autogenous bone marrow aspirate concentrate (BMAC).
Ten patients were distributed into two groups ( = 5), according to the type of grafting material used: Control group (CG), particulate bone xenograft alone, and test group (TG), particulate bone xenograft combined with BMAC. A bone specimen was removed from the graft area 4 months after grafting, before implant placement. The specimens were processed and submitted to immunohistochemical analysis for detection of OC and Runx2. Histomorphometry was used to ascertain the percentage of stained areas in both groups. The Wilcoxon Mann-Whitney -Test was used in the statistical analysis ( < 0.05).
The immunohistochemical analysis revealed a significantly higher OC expression in the TG than in the CG, namely 27.40 ± 1.34% and 11.40 ± 2.70%, respectively ( < 0.05), and a significantly higher Runx2 expression in the TG than in the CG, namely 2.80 ± 0.84% and 0.40 ± 0.55%, respectively ( < 0.05).
The OC and Runx2 expression levels were higher when BMAC was associated with the bone xenograft than when it was not.
已知大量参与骨生成的介质可影响骨骼发育和修复；然而，这些介质是否可作为骨成熟的标志物尚未确定。
评估在使用或不使用自体骨髓抽吸浓缩物（BMAC）联合颗粒状骨异种移植物重建萎缩性上颌前骨过程中获取的骨活检标本中骨钙素（OC）和Runt相关转录因子2（Runx2）的表达情况。
根据所使用的移植材料类型，将10例患者分为两组（每组n = 5）：对照组（CG），仅使用颗粒状骨异种移植物；试验组（TG），颗粒状骨异种移植物联合BMAC。在植入种植体前，于移植后4个月从移植区域取出骨标本。对标本进行处理并进行免疫组织化学分析以检测OC和Runx2。使用组织形态计量学确定两组中染色区域的百分比。采用Wilcoxon Mann-Whitney检验进行统计分析（P < 0.05）。
免疫组织化学分析显示，试验组中OC的表达明显高于对照组，分别为27.40 ± 1.34%和11.40 ± 2.70%（P < 0.05），试验组中Runx2的表达也明显高于对照组，分别为2.80 ± 0.84%和0.40 ± 0.55%（P < 0.05）。
与未联合BMAC时相比，BMAC与骨异种移植物联合使用时OC和Runx2表达水平更高。
