College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, 116029, China.
College of Pharmacy, Dalian Medical University, Dalian, 116044, China.
Chem Biodivers. 2022 Nov;19(11):e202200345. doi: 10.1002/cbdv.202200345. Epub 2022 Nov 8.
It has been reported that intestinal ischemia-reperfusion injury (IIRI) is closely related to inflammatory response, apoptosis and oxidative stress. Ellagic acid (EA) has been proved to have antioxidant and anti-inflammatory effects and can inhibit tumor angiogenesis. The purpose of this study was to investigate the protective effects of EA on IIRI in mice.
A mouse model of IIRI was established by clamping the mesenteric artery. Effects and mechanisms of EA on IIRI were investigate by transcriptomics combined with functional experiments.
The symptoms of IIRI were reflected in significant increases in inflammatory factors such as TNF-α and IL-1β; significant increases in oxidative stress indicators such as MDA and GSH and decreases in SOD and promotion of the apoptotic protein Bax/Bcl-2. These indicators were significantly alleviated by EA. And after EA treatment, transcriptomics results identified AKT1 differentially expressed mRNAs mainly enriched in PI3K/AKT signalling pathway.
This study illustrates the protective effects against IIRI, the possible mechanisms were also studied. This study provides new scientific information for the application of EA in IIRI therapy.
据报道，肠缺血再灌注损伤（IIRI）与炎症反应、细胞凋亡和氧化应激密切相关。鞣花酸（EA）已被证明具有抗氧化和抗炎作用，并能抑制肿瘤血管生成。本研究旨在探讨 EA 对小鼠 IIRI 的保护作用。
通过夹闭肠系膜动脉建立小鼠 IIRI 模型。通过转录组学结合功能实验研究 EA 对 IIRI 的作用及机制。
IRI 的症状表现在 TNF-α和 IL-1β等炎症因子显著增加；MDA 和 GSH 等氧化应激指标显著升高，SOD 降低，促凋亡蛋白 Bax/Bcl-2 增加。这些指标均被 EA 显著缓解。经 EA 处理后，转录组学结果鉴定出 AKT1 差异表达的 mRNAs 主要富集在 PI3K/AKT 信号通路中。
本研究阐明了 EA 对 IIRI 的保护作用及其可能的机制，为 EA 在 IIRI 治疗中的应用提供了新的科学信息。
