Department of Abdominal Transplant Surgery & Transplant Coordination, University Hospitals Leuven, 3000 Leuven, Belgium.
Abdominal Transplant Laboratory, Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.
Int J Mol Sci. 2023 Jan 30;24(3):2587. doi: 10.3390/ijms24032587.
Animal research in intestinal ischemia-reperfusion injury (IRI) is mainly performed in rodent models. Previously, intraperitoneal (I.P.) injections with ketamine-xylazine mixtures were used. Nowadays, volatile anesthetics (isoflurane) are more common. However, the impact of the anesthetic method on intestinal IRI has not been investigated. We aim to analyze the different anesthetic methods and their influence on the extent of intestinal IRI in a rat model. Male Sprague-Dawley rats were used to investigate the effect of I.P. anesthesia on 60 min of intestinal ischemia and 60 min of reperfusion in comparison to hyperoxygenation (100% O) and volatile isoflurane anesthesia. In comparison to I.P. anesthesia with room air (21% O), supplying 100% O improved 7-day survival by cardiovascular stabilization, reducing lactic acidosis and preventing vascular leakage. However, this had no effect on the intestinal epithelial damage, permeability, and inflammatory response observed after intestinal IRI. In contrast to I.P. + 100% O, isoflurane anesthesia reduced intestinal IRI by preventing ongoing low-flow reperfusion hypotension, limiting intestinal epithelial damage and permeability, and by having anti-inflammatory effects. When translating the aforementioned results of this study to clinical situations, such as intestinal ischemia or transplantation, the potential protective effects of hyperoxygenation and volatile anesthetics require further research.
动物在肠缺血再灌注损伤（IRI）的研究主要在啮齿类动物模型中进行。先前，常使用腹腔内（I.P.）注射氯胺酮-甲苯噻嗪混合物。如今，挥发性麻醉剂（异氟烷）更为常用。然而，麻醉方法对肠 IRI 的影响尚未被研究。我们旨在分析不同的麻醉方法及其对大鼠模型中肠 IRI 程度的影响。雄性 Sprague-Dawley 大鼠用于研究 I.P. 麻醉对 60 分钟肠缺血和 60 分钟再灌注的影响，并与高氧（100% O）和挥发性异氟烷麻醉进行比较。与 I.P. 麻醉用空气（21% O）相比，提供 100% O 通过心血管稳定来提高 7 天存活率，减少酸中毒并防止血管渗漏。然而，这对肠 IRI 后观察到的肠上皮损伤、通透性和炎症反应没有影响。与 I.P. + 100% O 相比，异氟烷麻醉通过防止再灌注低血压、限制肠上皮损伤和通透性以及具有抗炎作用来减轻肠 IRI。当将本研究的上述结果转化为临床情况，如肠缺血或移植时，需要进一步研究高氧和挥发性麻醉剂的潜在保护作用。
