Piskorz Ewa W, Xu Linhao, Ma Yingrui, Jiang Hua
Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
J Exp Bot. 2023 Feb 5;74(3):835-847. doi: 10.1093/jxb/erac397.
Doubled haploids (DHs) fix traits from hybrids in one generation. DH induction includes two changes in ploidy levels typically associated with variation in DNA methylation. However, DNA methylation patterns in DH plants and their biological significance are largely unknown. We generated three DH lines in Arabidopsis thaliana by crossing a haploid inducer with the accession Col-0, thus removing tissue culture and hybridization as a variable. DH induction produced thousands of differentially DNA methylated regions (DMRs), most of which were stochastic. Both haploidization and colchicine-induced genome duplication produced DMRs; the former mainly yielded DMRs at non-CG contexts, whereas the latter affected differential gene body methylation. Spontaneous genome doubling of haploid plants also induced DMRs in greater numbers than self-propagation. Our results provide the first evidence that haploid induction and genome doubling result in differential DNA methylation, offering a novel approach to induce epialleles.
双单倍体（DHs）能在一代中固定杂种的性状。DH诱导包括通常与DNA甲基化变化相关的两个倍性水平变化。然而，DH植物中的DNA甲基化模式及其生物学意义在很大程度上尚不清楚。我们通过将单倍体诱导系与Col-0生态型杂交，在拟南芥中产生了三个DH系，从而消除了组织培养和杂交这两个变量。DH诱导产生了数千个差异DNA甲基化区域（DMR），其中大多数是随机的。单倍体化和秋水仙碱诱导的基因组加倍都产生了DMR；前者主要在非CG背景下产生DMR，而后者影响基因体差异甲基化。单倍体植物的自发基因组加倍也比自交繁殖诱导出更多的DMR。我们的结果首次证明单倍体诱导和基因组加倍会导致差异DNA甲基化，为诱导表观等位基因提供了一种新方法。
