Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China.
Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China.
Poult Sci. 2022 Dec;101(12):102187. doi: 10.1016/j.psj.2022.102187. Epub 2022 Sep 16.
Our previous study has demonstrated that administration of ginsenoside Rg3 ameliorates immune stress by inhibiting inflammatory responses, reducing oxidative damage and upregulating mRNA expression of mTOR, SOD-1, and HO-1. However, the specific mechanism in relation to the protective effect of ginsenoside Rg3 on stressed broilers especially the metabolites alteration remains obscure. The present study aimed to investigate the underlined mechanism in relation to the pathogenesis and protective effect of ginsenoside Rg3 on stressed broilers using liquid chromatograph-mass spectrometry profiling. Eighteen broiler chicks were randomly allocated to 3 treatments: Control, Model and Rg3. Chickens in Rg3 group received intraperitoneally administered 1 mg/kg Rg3 2 h before LPS challenge. Then the broilers were intraperitoneally injection of 250 µg/kg LPS at the age of 12, 14, 33, and 35 d to induce immune stress. Control group was injected with an equivalent amount of sterile saline. At the end of the experiment, the serum was obtained for metabolomics analysis. The changes in serum metabolic profiles were investigated with the application of metabolomics approach. Distinct changes in metabolite patterns in serum were observed by orthogonal partial least square-discriminate analysis. In total, 35 metabolites were identified, among which 17 differential metabolites were found between Control and Model group, and 18 differential metabolites were identified between Model and Rg3 group. Metabolic pathway analysis revealed potential serum metabolites involved in oxidative stress and inflammation, degradation of lipid and protein in broiler chicks with immune stress. In addition, the protective effect of Rg3 on the stressed chicks may be largely mediated by BCAA metabolism, apoptosis and mTOR signaling pathway. These results suggested the potential biomarkers involved in pathogenesis and prevention of stress induced by Escherichia coli lipopolysaccharide.
我们之前的研究表明，人参皂苷 Rg3 通过抑制炎症反应、减少氧化损伤以及上调 mTOR、SOD-1 和 HO-1 的 mRNA 表达来改善免疫应激。然而，关于人参皂苷 Rg3 对应激肉鸡的保护作用的具体机制，特别是代谢物的变化仍然不清楚。本研究旨在使用液相色谱-质谱分析技术研究人参皂苷 Rg3 对应激肉鸡发病机制和保护作用的潜在机制。将 18 只肉鸡随机分为 3 组：对照组、模型组和 Rg3 组。Rg3 组在 LPS 攻毒前 2 小时腹腔注射 1mg/kg Rg3。然后，在 12、14、33 和 35 日龄时，将鸡腹腔注射 250µg/kg LPS 以诱导免疫应激。对照组注射等量无菌生理盐水。实验结束时，采集血清进行代谢组学分析。应用代谢组学方法研究血清代谢谱的变化。通过正交偏最小二乘法判别分析观察到血清代谢模式的明显变化。共鉴定出 35 种代谢物，其中对照组和模型组之间有 17 种差异代谢物，模型组和 Rg3 组之间有 18 种差异代谢物。代谢途径分析显示，潜在的血清代谢物参与了免疫应激肉鸡的氧化应激和炎症、脂质和蛋白质的降解。此外，Rg3 对应激雏鸡的保护作用可能主要通过支链氨基酸代谢、细胞凋亡和 mTOR 信号通路来介导。这些结果表明了与大肠杆菌脂多糖诱导的应激相关的潜在发病机制和预防的生物标志物。
