Sealy-Lewis H M, Scazzocchio C, Lee S
Mol Gen Genet. 1978 Sep 8;164(3):303-8. doi: 10.1007/BF00333161.
In Aspergillus nidulans uric acid can be produced from xanthine via purine hydroxylase I (xanthine dehydrogenase) or via the xanthine alternative pathway (Darlington and Scazzocchio, Biochem. Biophys. Acta, 166, 569--571; 1968). A mutation defective in the xanthine alternative pathway of Aspergillus nidulans is described. By combining this mutation with hxB-20 which results in complete loss of purine hydroxylase I and II activities, but which conserves cross-reacting material, it is possible to block completely uric acid production and thus investigate which are the effective in vivo inducers of three enzymes under the control of the positive regulatory gene uaY: adenine deaminase, purine hydroxylase I (measured as cross-reacting material) and urate oxidase. It is concluded that uric acid is the only effective physiological inducer, while its 2 and 8 thio-analogues serve as gratuitous inducers.
在构巢曲霉中，尿酸可以通过嘌呤羟化酶I（黄嘌呤脱氢酶）或通过黄嘌呤替代途径由黄嘌呤产生（达林顿和斯卡佐基奥，《生物化学与生物物理学学报》，166，569 - 571；1968年）。本文描述了构巢曲霉黄嘌呤替代途径中的一个缺陷突变。通过将此突变与hxB - 20相结合（hxB - 20会导致嘌呤羟化酶I和II的活性完全丧失，但保留交叉反应物质），就有可能完全阻断尿酸的产生，从而研究在正调控基因uaY控制下的三种酶：腺嘌呤脱氨酶、嘌呤羟化酶I（以交叉反应物质来衡量）和尿酸氧化酶，在体内有效的诱导物是什么。得出的结论是，尿酸是唯一有效的生理性诱导物，而其2位和8位硫类似物作为安慰诱导物。
