Vertuan Mariele, França da Silva Júlia, Ferreira Amanda Moura, Braga Aline Silva, Magalhães Ana Carolina
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Caries Res. 2022;56(5-6):447-454. doi: 10.1159/000527388. Epub 2022 Oct 10.
This study evaluated the remineralizing effect of a toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler on demineralized enamel in situ. For this, 180 bovine enamel samples were demineralized by using a microcosm biofilm model for 3 days. Thereafter, the samples were randomly signed to 15 healthy volunteers and to 3 cross-over in situ phases corresponding to the following treatments: (1) toothpaste containing 1,500 ppm F as NaMFP (positive control, Colgate®Cavity Protection), (2) toothpaste containing 5% S-PRG filler (Shofu®), and (3) placebo toothpaste (negative control prepared by Shofu®). Four demineralized enamel blocks were fixed into each palatal appliance per phase. The volunteers wore the appliances for 5 days and were trained to brush their teeth 2 times for 2 min a day, while one drop of the toothpaste's slurry (1:3) was dripped on each sample for the same period. The surface hardness and TMR analyses were done and analyzed by ANOVA/Tukey and t test (p < 0.05). S-PRG filler and Colgate® toothpastes were equally able to improve 2-2.5× enamel remineralization by the analysis of % surface hardness recovery. However, S-PRG toothpaste was the only one able to significantly improve ΔΔZ (the integrated mineral loss recovery: 1,489 ± 503 %vol.μm) compared to placebo (1,050 ± 467 %vol.μm), while Colgate® did not differ from placebo. No differences were seen between the groups with respect to ΔLD. S-PRG filler and Colgate® toothpastes show similar potential to remineralize the lesion surface. However, S-PRG toothpaste is better to recover mineral loss at the subsurface area.
本研究评估了一种含有表面预反应玻璃离子（S-PRG）填料的牙膏对原位脱矿釉质的再矿化作用。为此，使用微观生物膜模型将180个牛牙釉质样本脱矿3天。此后，将样本随机分配给15名健康志愿者，并进行3个交叉原位阶段的实验，对应以下处理：（1）含1500 ppm氟的氟化钠单氟磷酸钠牙膏（阳性对照，高露洁®防蛀牙膏），（2）含5% S-PRG填料的牙膏（松风®），（3）安慰剂牙膏（由松风®制备的阴性对照）。每个阶段，将4个脱矿釉质块固定在每个腭部矫治器中。志愿者佩戴矫治器5天，并接受每天刷牙2次、每次2分钟的训练，同时在同一时期，将一滴牙膏浆液（1:3）滴在每个样本上。进行表面硬度和TMR分析，并通过方差分析/图基检验和t检验进行分析（p < 0.05）。通过分析表面硬度恢复百分比，S-PRG填料牙膏和高露洁®牙膏在提高釉质再矿化2 - 2.5倍方面效果相当。然而，与安慰剂（1050 ± 467 %vol.μm）相比，S-PRG牙膏是唯一能够显著提高ΔΔZ（累积矿物质损失恢复：1489 ± 503 %vol.μm）的牙膏，而高露洁®牙膏与安慰剂无差异。各实验组在ΔLD方面未见差异。S-PRG填料牙膏和高露洁®牙膏在使病变表面再矿化方面显示出相似的潜力。然而，S-PRG牙膏在恢复亚表面区域的矿物质损失方面效果更好。
