Department of Pediatric Dentistry and Public Health, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil,
Department of Pediatric Dentistry and Public Health, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
Caries Res. 2019;53(3):260-267. doi: 10.1159/000491555. Epub 2018 Oct 2.
To evaluate the remineralizing potential of a conventional toothpaste (1,100 ppm F) supplemented with nano-sized sodium hexametaphosphate (HMPnano) in artificial caries lesions in situ.
This double-blinded crossed study was performed in 4 phases of 3 days each. Twelve subjects used palatal appliances containing 4 bovine enamel blocks with artificial caries lesions. Volunteers were randomly assigned into the following treatment groups: no F/HMP/HMPnano (Placebo); 1,100 ppm F (1100F); 1100F plus 0.5% micrometric HMP (1100F/HMP) and 1100F plus 0.5% nano-sized HMP (1100F/HMPnano). Volunteers were instructed to brush their natural teeth with the palatal appliances in the mouth for 1 min (3 times/day), so that blocks were treated with natural slurries of toothpastes. After each phase, surface hardness post-remineralization (SH2), integrated recovery of subsurface hardness (ΔIHR), integrated mineral recovery (ΔIMR) and enamel F concentration were determined. Data were submitted to analysis of variance and Student-Newman-Keuls' test (p < 0.001).
Enamel surface became 42% harder when treated with 1100F/HMPnano in comparison with 1100F (p < 0.001). Treatment with 1100F/HMP and 1100F/HMPnano promoted an increase of ∼23 and ∼87%, respectively, in ΔIHR when compared to 1100F (p < 0.001). In addition, ΔIMR for the 1100F/HMPnano was ∼75 and ∼33% higher when compared to 1100F and 1100F/HMP respectively (p < 0.001). Enamel F uptake was similar among all groups except for the placebo (p < 0.001).
The addition of 0.5% HMPnano to a conventional fluoride toothpaste was able to promote an additional remineralizing effect of artificial caries lesions.
评估常规含氟牙膏（1100ppmF）中添加纳米六偏磷酸钠（HMPnano）对人工龋损的再矿化潜力。
本双盲交叉研究共分为 4 个阶段，每个阶段持续 3 天。12 名受试者使用含有 4 块牛牙釉质块的腭托，每个釉质块均有 1 个人工龋损。志愿者随机分为以下治疗组：无氟/六偏磷酸钠/纳米六偏磷酸钠（安慰剂）；1100ppmF（1100F）；1100F 加 0.5%微米级六偏磷酸钠（1100F/HMP）和 1100F 加 0.5%纳米级六偏磷酸钠（1100F/HMPnano）。志愿者被指导用腭托在口腔中刷牙 1 分钟（每天 3 次），以便用牙膏的天然糊剂处理牙釉质块。每个阶段后，测定表面再矿化后硬度（SH2）、表面下硬度综合恢复（ΔIHR）、整体矿化恢复（ΔIMR）和牙釉质 F 浓度。数据采用方差分析和 Student-Newman-Keuls 检验（p＜0.001）。
与 1100F 相比，用 1100F/HMPnano 处理后牙釉质表面硬度增加了 42%（p＜0.001）。与 1100F 相比，用 1100F/HMP 和 1100F/HMPnano 处理后，ΔIHR 分别增加了约 23%和 87%（p＜0.001）。此外，与 1100F 和 1100F/HMP 相比，1100F/HMPnano 的ΔIMR 分别提高了约 75%和 33%（p＜0.001）。除了安慰剂组（p＜0.001）外，各组的牙釉质 F 摄取量均相似。
在常规含氟牙膏中添加 0.5%纳米六偏磷酸钠可对人工龋损产生额外的再矿化作用。
