母体血浆外泌体 miRNA 的改变揭示了母体循环与胎盘之间的选择性物质交换。
Alterations in maternal plasma exosomal miRNAs revealed selective material exchange between maternal circulation and placenta.
机构信息
Department of Fetal Medicine & Prenatal Diagnosis Center, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, PR China.
Clinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, PR China.
出版信息
J Obstet Gynaecol Res. 2023 Jan;49(1):109-121. doi: 10.1111/jog.15452. Epub 2022 Oct 10.
AIM
Exosomes have emerged as important regulators in the communication between maternal peripheral blood and placenta. We aimed to compare maternal plasma exosomal miRNAs profile between healthy pregnant and nonpregnant women, screen for differential expressed miRNAs and their potential regulatory role during pregnancy.
METHODS
We isolated exosomes from plasma of mid-trimester, last trimester, and nonpregnant women (n = 6 each group), analyzed the miRNA profile using next-generation sequencing.
RESULTS
Several miRNA clusters were expressed in plasma exosomes, such as C19MC, C14MC, and let-7 family, miRNAs in each cluster may have synergistic effect during pregnancy. We assumed maternal circulating exosomal miRNA could be transported into placenta or selectively uptook by placenta, which was consistent with the fact that many pregnancy-associated or placenta highly expressed miRNAs reduced in exosomes during pregnancy. Some exosomal miRNAs were mainly secreted by the placenta, which could act as markers that reflect changes in the function and microenvironment of the placenta.
CONCLUSIONS
Exosomal miRNAs are associated with placenta development and have potential as molecular markers.
目的
外泌体已成为母体外周血与胎盘之间通讯的重要调节因子。本研究旨在比较健康孕妇和非孕妇的母体外周血血浆外泌体 miRNA 谱，筛选差异表达的 miRNA 及其在妊娠期间的潜在调节作用。
方法
我们从中孕期、晚孕期和非孕期妇女的血浆中分离外泌体（每组 n=6），并使用下一代测序分析 miRNA 谱。
结果
一些 miRNA 簇在血浆外泌体中表达，如 C19MC、C14MC 和 let-7 家族，每个簇中的 miRNA 可能在妊娠期间具有协同作用。我们假设母体外周循环的外泌体 miRNA 可以被转运到胎盘或被胎盘选择性摄取，这与妊娠期间胎盘高表达或与妊娠相关的 miRNA 在 exosomes 中减少的事实是一致的。一些外泌体 miRNA 主要由胎盘分泌，可作为反映胎盘功能和微环境变化的标志物。
结论
外泌体 miRNA 与胎盘发育有关，具有作为分子标志物的潜力。
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