Hannon Edward, Pellegrini Marco, Scottoni Federico, Durkin Natalie, Shibuya Soichi, Lutman Roberto, Proctor Toby J, Hutchinson J Ciaran, Arthurs Owen J, Phylactopoulos Demetra-Ellie, Maughan Elizabeth F, Butler Colin R, Eaton Simon, Lowdell Mark W, Bonfanti Paola, Urbani Luca, De Coppi Paolo
Great Ormond Street Institute of Child Health, University College London, London WC1N 1EH, UK.
Department of Paediatric Surgery, Leeds Children's Hospital, Leeds Teaching Hospitals NHS Trust, Leeds LS1 3EX, UK.
iScience. 2022 Sep 22;25(10):105174. doi: 10.1016/j.isci.2022.105174. eCollection 2022 Oct 21.
Decellularization of esophagi from several species for tissue engineering is well described, but successful implantation in animal models of esophageal replacement has been challenging. The purpose of this study was to assess feasibility and applicability of esophageal replacement using decellularized porcine esophageal scaffolds in a new pre-clinical model. Following surgical replacement in rabbits with a vascularizing muscle flap, we observed successful anastomoses of decellularized scaffolds, cues of early neovascularization, and prevention of luminal collapse by the use of biodegradable stents. However, despite the success of the surgical procedure, the long-term survival was limited by the fragility of the animal model. Our results indicate that transplantation of a decellularized porcine scaffold is possible and vascular flaps may be useful to provide a vascular supply, but long-term outcomes require further pre-clinical testing in a different large animal model.
对多种物种的食管进行脱细胞处理以用于组织工程已有详细描述，但在食管置换动物模型中的成功植入一直具有挑战性。本研究的目的是在一种新的临床前模型中评估使用脱细胞猪食管支架进行食管置换的可行性和适用性。在用血管化肌瓣对兔子进行手术置换后，我们观察到脱细胞支架成功吻合、早期新血管形成的迹象，以及使用可生物降解支架防止管腔塌陷。然而，尽管手术过程成功，但动物模型的脆弱性限制了长期存活。我们的结果表明，脱细胞猪支架的移植是可行的，血管瓣可能有助于提供血管供应，但长期结果需要在不同的大型动物模型中进行进一步的临床前测试。
