Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 215123 Suzhou, China.
Biomater Sci. 2022 Oct 25;10(21):6146-6159. doi: 10.1039/d2bm01244h.
To optimally apply antibiotics and antimicrobials, smart wound dressing conferring controlled drug release and preventing adhesions of biological objects is advantageous. Poly(-isopropylacrylamide) (PNIPAAm), a conventional thermo-responsive polymer, and poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), a typical antifouling polymer, have therefore potential to be fabricated as copolymers to achieve dual functions of thermo-responsiveness and antifouling. Herein, a hydrogel made of PNIPAM--PMPC was designed and loaded with octenidine, a widely applied antimicrobial agent for wound treatment, to achieve both antifouling and triggered drug release. The thermo-switch of the fabricated hydrogel allowed 25-fold more octenidine release at 37 °C (infected wound temperature) than at 30 °C (normal skin temperature) after 120 minutes, which led to at least a 3 lg reduction of the viable bacteria at 37 °C on artificially infected wounds. Furthermore, we pioneeringly assessed the antifouling property of the material in PBS buffer using single molecule/cell/bacterial force spectroscopy, and revealed that the fabricated hydrogel displayed distinctive antifouling properties against proteins, mammalian cells, and bacteria. This work demonstrated a promising design of a hydrogel applicable for preventing and treating wound infections. The concept of dual-functional materials can be envisaged for other clinical applications related to the prevention of biofilm-associated infections, such as urinary catheters, stents, and dental implants.
为了优化抗生素和抗菌药物的应用，具有控制药物释放和防止生物物体粘连功能的智能伤口敷料具有优势。聚（异丙基丙烯酰胺）（PNIPAAm）是一种常规的温度响应性聚合物，聚（2-甲基丙烯酰氧乙基磷酸胆碱）（PMPC）是一种典型的抗污聚合物，因此有可能被制成共聚物，以实现温度响应性和抗污的双重功能。在此，设计了一种由 PNIPAM-PMPC 制成的水凝胶，并负载了广泛用于伤口治疗的抗菌剂奥替尼啶，以实现抗污和触发药物释放。所制备的水凝胶的温度开关允许在 120 分钟后，在 37°C（感染伤口温度）下释放 25 倍以上的奥替尼啶，而在 30°C（正常皮肤温度）下释放的奥替尼啶，这导致在人工感染的伤口上，37°C 时存活细菌至少减少了 3 lg。此外，我们开创性地使用单分子/细胞/细菌力谱法评估了该材料在 PBS 缓冲液中的抗污性能，并揭示了所制备的水凝胶对蛋白质、哺乳动物细胞和细菌具有独特的抗污性能。这项工作展示了一种有前途的水凝胶设计，适用于预防和治疗伤口感染。这种双功能材料的概念可以设想用于其他与预防生物膜相关感染相关的临床应用，如导尿管、支架和牙种植体。
