Maier P, Manser P, Zbinden G
Mutat Res. 1978 Oct;54(2):159-65. doi: 10.1016/0165-1161(78)90036-5.
Growth of granulation tissue was induced in rats inside a subcutaneous air pouch by injection of croton oil. Granulation tissue, isolated and cultured in vitro, gave satisfactory and reproducible cloning efficiency of fibroblast-like cells. This experimental model system was used to study the induction of autosomal point mutations in vivo leading to ouabain resistance. For this purpose the mutagen MNNG was administered in the granuloma pouch, and the formation of ouabain-resistant clones was determined in vitro. Various application schedules, expression times in vivo and selective conditions in vitro were evaluated. The highest frequencies of ouabain-resistant clones were found when MNNG was injected into the pouch 24--48 h after induction of granulation tissue, followed by an expression time in vivo of 24--48 h. No ouabain-resistant clones were formed by cells isolated from untreated rats or from animals receiving the highest tolerated doses of MNNG per os or by intraperitoneal injection. The potential usefulness of the granuloma pouch assay for the evaluation of mutagenic and carcinogenic substances in vivo is discussed.
通过注射巴豆油在大鼠皮下气囊内诱导肉芽组织生长。分离并在体外培养的肉芽组织，其成纤维细胞样细胞的克隆效率令人满意且可重复。该实验模型系统用于研究体内常染色体点突变导致哇巴因抗性的诱导情况。为此，将诱变剂N-甲基-N'-硝基-N-亚硝基胍（MNNG）施用于肉芽肿囊，并在体外测定哇巴因抗性克隆的形成。评估了各种应用方案、体内表达时间和体外选择条件。当在肉芽组织诱导后24 - 48小时将MNNG注入囊中，随后体内表达时间为24 - 48小时时，发现哇巴因抗性克隆的频率最高。从未经处理的大鼠或经口或腹腔注射接受最高耐受剂量MNNG的动物分离的细胞未形成哇巴因抗性克隆。讨论了肉芽肿囊试验在体内评估诱变和致癌物质方面的潜在用途。
