Jaggi W, Lutz W K, Schlatter C
Experientia. 1979 May 15;35(5):631-2. doi: 10.1007/BF01960365.
The covalent binding of tritiated benzo(a)pyrene (BP) to DNA has been determined in rat liver in vivo, in rat liver perfused in situ, after incubation of BP with liver single cells, with liver homogenate, with liver microsomes and DNA, with fibroblasts from a rat granuloma pouch, and with 2 cell lines. Liver single cells were found to be a valuable compromise between the most sensitive system (microsomal incubation of BP and DNA) and the biologically most relevant system (in vivo).
已在以下几种情况下测定了氚标记的苯并(a)芘（BP）与DNA的共价结合：大鼠体内的肝脏、原位灌注的大鼠肝脏、BP与肝脏单细胞、肝脏匀浆、肝脏微粒体和DNA、大鼠肉芽肿袋中的成纤维细胞以及两种细胞系共同孵育后。结果发现，肝脏单细胞是最敏感系统（BP与DNA的微粒体孵育）和生物学上最相关系统（体内）之间的一种有价值的折中选择。
