Versura P, Maltarello M C
Curr Eye Res. 1987 Jul;6(7):943-6. doi: 10.3109/02713688709034863.
A new schedule in the processing of conjunctival biopsies for ultrastructural investigations is proposed. The specimens are first examined in Scanning Electron Microscopy (SEM), briefly rehydrated and secondly processed for observation in Transmission Electron Microscopy (TEM). The rehydration counterbalances the shrinkage due to the previous processing steps applied for SEM examination and allows a good TEM morphology of the recovered specimens to be achieved. The correlative SEM/TEM study on the same conjunctival biopsy provides complete informations on both the surface and the inner structure of the tissue. This appears of value in the investigation of conjunctival biopsy from eyes affected by tear film quantitative and/or qualitative alterations.
本文提出了一种用于超微结构研究的结膜活检标本处理新方案。标本首先在扫描电子显微镜（SEM）下检查，然后短暂复水，接着进行处理以用于透射电子显微镜（TEM）观察。复水可抵消因先前用于SEM检查的处理步骤而导致的收缩，并能使回收标本获得良好的TEM形态。对同一结膜活检标本进行的相关SEM/TEM研究可提供有关组织表面和内部结构的完整信息。这在对受泪膜定量和/或定性改变影响的眼部结膜活检研究中似乎具有重要价值。
