Pontificia Universidad Javeriana, Bogotá, Colombia; Universidad Antonio Nariño, Sede Circunvalar, Bogotá, Colombia.
Pontificia Universidad Javeriana, Bogotá, Colombia.
Mar Pollut Bull. 2022 Nov;184:114204. doi: 10.1016/j.marpolbul.2022.114204. Epub 2022 Oct 8.
Mangroves are often exposed to heavy metals that accumulate in the food chain, generate toxicity to mangrove plants and affect microbial diversity. This study determined the abundance of genes associated with resistance and tolerance to heavy metals in the rhizosphere microbiome of Avicennia germinans from a semi-arid mangrove of La Guajira-Colombia by metagenomics and genomics approach. Twenty-eight genes associated with tolerance and 49 genes related to resistance to heavy metals were detected. Genes associated with tolerance and resistance to Cu, especially cusA and copA, were the most abundant. The highest number of genes for tolerance and resistance were for Zn and Co, respectively. The isolate Vibrio fluvialis showed the ability to tolerate Cu, Ni, Zn, and Cd. This work used a complementary approach of metagenomics and genomics to characterize the potential of mangrove microorganisms to tolerate and resist heavy metals and the influence of salinity on their abundance.
红树林经常暴露在食物链中积累的重金属中，对红树林植物产生毒性，并影响微生物多样性。本研究通过宏基因组学和基因组学方法，确定了哥伦比亚拉瓜希拉半干旱红树林中海桑根际微生物组中与重金属抗性和耐受性相关的基因丰度。检测到与耐受性相关的 28 个基因和与重金属抗性相关的 49 个基因。与 Cu 耐受性和抗性相关的基因，尤其是 cusA 和 copA，最为丰富。对 Zn 和 Co 的耐受性和抗性的基因数量最多。分离出的黄杆菌表现出耐受 Cu、Ni、Zn 和 Cd 的能力。这项工作使用宏基因组学和基因组学的互补方法来描述红树林微生物耐受和抵抗重金属的潜力，以及盐度对其丰度的影响。
