Doolan D L, Ward L C
Cytobios. 1987;51(204):49-61.
The incorporation of 14C-tyrosine into protein of incubated Ehrlich ascites tumour cells was measured over a 10-fold range of external amino acid concentrations, the composition of which simulated that of mouse intraperitoneal fluid. Incorporation was linear with time and the rate of incorporation increased with increasing external amino acid concentration up to at least six times intraperitoneal concentrations. Further increases in external amino acid supply did not yield a concomitant increase in the rate of tyrosine incorporation, a maximum fractional protein synthetic rate of approximately 100% d-1 being achieved. The specific activity of tyrosine in intracellular fluid was approximately 95% of that of extracellular fluid, irrespective of the external amino acid concentration.
在10倍范围的外部氨基酸浓度下，测定了14C-酪氨酸掺入培养的艾氏腹水瘤细胞蛋白质中的情况，其组成模拟了小鼠腹腔液的组成。掺入与时间呈线性关系，掺入速率随着外部氨基酸浓度的增加而增加，直至至少为腹腔内浓度的六倍。外部氨基酸供应的进一步增加并未导致酪氨酸掺入速率随之增加，达到了约100% d-1的最大蛋白质合成分数速率。无论外部氨基酸浓度如何，细胞内液中酪氨酸的比活性约为细胞外液的95%。
