Doolan D L, Ward L C
Cytobios. 1987;51(204):7-23.
The uptake and intracellular accumulation of an amino acid mixture by incubated Ehrlich ascites tumour cells was studied. The composition of the amino acid mixture simulated that of mouse intraperitoneal fluid and amino acid uptake was studied over a range of concentrations between 0.0 (no added amino acids) and 10.0-times intraperitoneal concentrations. For most amino acids uptake into cells and intracellular accumulation occurred as concentrations were increased up to 6.0-times the intraperitoneal concentrations; further increases in external amino acid concentrations did not increase concomitantly with intracellular concentrations. These data, when analysed indicated a net protein synthetic rate of 20% d-1 and that the rate of protein synthesis may be limited by the availability of the amino acids lysine, threonine and methionine.
研究了经孵育的艾氏腹水瘤细胞对氨基酸混合物的摄取和细胞内积累情况。该氨基酸混合物的组成模拟了小鼠腹腔液的组成，并在0.0（不添加氨基酸）至腹腔浓度10.0倍的一系列浓度范围内研究了氨基酸摄取。对于大多数氨基酸，随着浓度增加至腹腔浓度的6.0倍，细胞摄取和细胞内积累都会发生；外部氨基酸浓度的进一步增加并不会使细胞内浓度随之增加。对这些数据进行分析时发现，净蛋白质合成率为20% d-1，并且蛋白质合成速率可能受赖氨酸、苏氨酸和蛋氨酸等氨基酸可用性的限制。
