Department of Genetics, Faculty of Agriculture, Cairo University, Cairo University, Giza, 12613, Egypt.
Department of Zoology and Agricultural Nematology Department, Faculty of Agriculture, Cairo University, Giza, P. O. Box, 12613, Egypt.
Exp Parasitol. 2022 Dec;243:108402. doi: 10.1016/j.exppara.2022.108402. Epub 2022 Oct 8.
Meloidogyne incognita leads to considerable losses in crop productivity. In this study, the impact of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) in 100, 200, or 300 ppm concentrations were investigated on some essential genes of M. incognita in vitro. For this purpose, AgNPs and ZnONPs were synthesized and characterized for their physicochemical properties. Thereafter, second-stage Juveniles (J2) of M. incognita were exposed to AgNPs and ZnONPs solution for 24 h. The LC, LC and mortality rates were calculated for both nanoparticles. Finally, the expression of parasitism genes (Xyl-1; msp-20; 16D10), neuropeptidergic gene (Ace-2), expansion-like proteins (MAP-1), and oxidative stress gene (GSTS-1) was analyzed. The results showed a successful preparation of nanoparticles to obtain a pure, well-dispersed, and stable suspension, as revealed by physicochemical properties. ZnONPs showed LC and LC values of 63.56 and 208.5 ppm, respectively, while AgNPs recorded 11.78 and 28.59 ppm, respectively. AgNPs at concentrations 100, 200, and 300 ppm showed mortality rate 66%, 84%, and 100%, respectively, whereas ZnONPs at the same concentrations caused a 58%, 78%, and 91% mortality rate, respectively. Analysis of gene expression showed dose-dependent downregulation of each parasitism gene Xyl-1, 16D10, and msp-20 genes, neuropeptidergic gene (Ace-2), and expansion-like proteins MAP-1 after treatment with either AgNPs or ZnONPs. On the other hand, the oxidative stress response gene GSTS-1 showed upregulation with all concentrations of AgNPs and ZnONPs. The study concluded that the AgNPs and ZnONPs have efficient nematocidal activity and can be used in Meloidogyne incognita control.
南方根结线虫会导致作物产量的大量损失。在这项研究中，研究了浓度为 100、200 或 300ppm 的银纳米颗粒（AgNPs）和氧化锌纳米颗粒（ZnONPs）对南方根结线虫一些必需基因的体外影响。为此，合成并表征了 AgNPs 和 ZnONPs 的物理化学性质。然后，将南方根结线虫的第二阶段幼虫（J2）暴露于 AgNPs 和 ZnONPs 溶液中 24 小时。计算了两种纳米颗粒的 LC、LC 和死亡率。最后，分析了寄生基因（Xyl-1；msp-20；16D10）、神经肽基因（Ace-2）、扩展样蛋白（MAP-1）和氧化应激基因（GSTS-1）的表达。结果表明，成功制备了纳米颗粒，获得了纯、分散良好且稳定的悬浮液，这是通过物理化学性质揭示的。ZnONPs 的 LC 和 LC 值分别为 63.56 和 208.5ppm，而 AgNPs 的 LC 和 LC 值分别为 11.78 和 28.59ppm。AgNPs 在 100、200 和 300ppm 浓度下的死亡率分别为 66%、84%和 100%，而 ZnONPs 在相同浓度下的死亡率分别为 58%、78%和 91%。基因表达分析表明，用 AgNPs 或 ZnONPs 处理后，每个寄生基因 Xyl-1、16D10 和 msp-20 基因、神经肽基因（Ace-2）和扩展样蛋白 MAP-1 的表达均呈剂量依赖性下调。另一方面，氧化应激反应基因 GSTS-1 在用所有浓度的 AgNPs 和 ZnONPs 处理后均上调。该研究得出结论，AgNPs 和 ZnONPs 具有有效的杀线虫活性，可用于防治南方根结线虫。
