开发一种可及且可扩展的定量聚合酶链反应检测方法，用于猴痘病毒检测。

Development of an Accessible and Scalable Quantitative Polymerase Chain Reaction Assay for Monkeypox Virus Detection.

机构信息

Department of Global Health, Milken Institute School of Public Health, George Washington University, Washington, District of Columbia, USA.

George Washington University Public Health Laboratory, School of Medicine and Health Sciences, George Washington University, Washington, District of Columbia, USA.

出版信息

J Infect Dis. 2023 Apr 26;227(9):1084-1087. doi: 10.1093/infdis/jiac414.

DOI:10.1093/infdis/jiac414

PMID:36221256

Abstract

During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard quantitative polymerase chain reaction (qPCR) diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with cycle threshold (Ct) values 17.46-35.59 and titer equivalents 8.01 × 106 to 2.45 × 100 plaque-forming unit (PFU)/mL. The detection limit was 3.59 PFU/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing MPX outbreak.

摘要

在 2022 年猴痘（MPX）疫情期间，检测受到限制且结果延迟，导致疫情持续传播。金标准实时聚合酶链反应（qPCR）诊断方法难以获得。本研究对 2022 年 6 月疾病预防控制中心（CDC）的 MPX qPCR 检测方法进行了调整，以便广泛实施。该方法在包含多种样本类型的基质中使用 MPX 库存进行了验证，检测到的循环阈值（Ct）值为 17.46-35.59，滴度等价物为 8.01×106 至 2.45×100 噬菌斑形成单位（PFU）/mL。检测限为 3.59 PFU/mL。敏感性和特异性均为 100%。该 qPCR 检测方法可在研究和公共卫生实验室中快速广泛实施，以在不断增长的 MPX 疫情中提高诊断能力。