Genomic Variation Laboratory, Department of Animal Science, University of California, Davis, Davis, CA, United States.
Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, United States.
J Hered. 2023 Mar 16;114(1):81-87. doi: 10.1093/jhered/esac056.
We present the reference genome of the Vernal Pool Fairy Shrimp Branchinecta lynchi. This branchiopod crustacean is endemic to California's freshwater ephemeral ponds. It faces enormous habitat loss and fragmentation as urbanization and agriculture have fundamentally changed the vernal pool landscape over the past 3 centuries. The assembled genome consists of 22 chromosome-length scaffolds that account for 96.85% of the total sequence. One hundred and ninety-five unscaffolded contigs comprise the rest of the genome's 575.6 Mb length. The genome is substantially complete with a BUSCO score of 90.0%. There is no immediately identifiable sex chromosome, typical for this class of organism. This new resource will permit researchers to better understand the adaptive capacity of this imperiled species, as well as answer lingering questions about anostracan physiology, sex determination, and development.
我们呈现了 Vernal Pool 仙女虾 Branchinecta lynchi 的参考基因组。这种介形纲甲壳动物是加利福尼亚淡水季节性池塘的特有物种。由于城市化和农业在过去 3 个世纪中从根本上改变了 Vernal Pool 景观，它面临着巨大的栖息地丧失和破碎化。组装的基因组由 22 个染色体长度的支架组成，占总序列的 96.85%。其余的基因组 575.6Mb 长度由 195 个未支架的连续序列组成。基因组完整性很高，BUSCO 评分为 90.0%。没有可立即识别的性染色体，这是此类生物体的典型特征。这个新资源将使研究人员能够更好地了解这个濒危物种的适应能力，并回答关于丰年虾生理学、性别决定和发育的悬而未决的问题。
