High-quality chromosome-level genome assembly of female reveals sex chromosome and gene organization.

is a crustacean genus belonging to the order Anostraca in the class Branchiopoda and lives in inland hypersaline lakes. Among the genus, is a valuable species as a fish food in the aquaculture industry or as an aquatic model organism for toxicity tests. However, genomic data for remains incomplete. In this study, high-quality genome assembly at the chromosome level of female was conducted by combining various sequencing and assembly technologies. The final assembled genome was 1.27 Gb in length, containing 21 chromosomal scaffolds (>10 Mb). The scaffold N50 was 45.3 Mb, with a complete BUSCO value of 91.0 %, thereby confirming that a high-quality genome was assembled. Gene annotation shows that the genome contained 67.26 % of repetitive sequences, and a total of 26,923 protein-coding genes were predicted. Among the 21 chromosome-scale scaffolds, chromosome 1 was identified as a sex chromosome Z. Additionally, five contigs of putative W chromosome fragments and the candidate sex-determining genes were suggested. Ten homeobox () genes were identified in on the chromosome 14, which were in two subclusters with a large gap. gene organizations within 13 arthropods showed that four anostracans had conserved synteny. This study provides a new female genome with sex chromosome and the first complete genomic arrangement of the cluster in Anostraca. This study will be a useful genomic and genetic reference for understanding the evolution and development of .