Sakamoto H, Maclusky N J, Schwartz P E, Naftolin F, Phil D, Den K, Takagi S
Endocrinol Jpn. 1987 Apr;34(2):179-87. doi: 10.1507/endocrj1954.34.179.
A rapid method for simultaneous preparation of cytosol and nuclear estrogen (E) and progestin (P) receptors and their in vitro determination is described. The method was applied to several uterine or ovarian surgical specimens to evaluate their steroid hormone "dependence". The results suggest that low cytoplasmic E receptor levels (ERc) are associated with higher nuclear E receptor (ERn) levels but no apparent correlation was observed between PRc and ERn levels. The method appeared to be suitable for screening steroid hormone receptor content in tumor tissues and may provide better estimation of steroid dependence since both cytoplasmic and nuclear compartments can be studied simultaneously.
本文描述了一种同时制备胞质和核雌激素（E）及孕激素（P）受体并进行体外测定的快速方法。该方法应用于多个子宫或卵巢手术标本，以评估其对类固醇激素的“依赖性”。结果表明，低细胞质E受体水平（ERc）与较高的核E受体（ERn）水平相关，但PRc与ERn水平之间未观察到明显相关性。该方法似乎适用于筛查肿瘤组织中的类固醇激素受体含量，并且由于可以同时研究细胞质和细胞核部分，可能会提供对类固醇依赖性更好的评估。
