Castro-Jara Silvia, Antilef Bárbara, Osbén Carolina, Alcántara Raúl, Fraga Marco, Nova-Lamperti Estefanía, Sánchez-Sanhueza Gabriela
Department of Restorative Dentistry, Endodontic Discipline, Faculty of Dentistry, Universidad de Concepción, Concepción, Chile.
Molecular & Translational Immunology Laboratory, Department of Clinical Biochemistry and Immunology, School of Pharmacy, Universidad de Concepción, Concepción, Chile.
Int Endod J. 2023 Jan;56(1):80-91. doi: 10.1111/iej.13847. Epub 2022 Oct 25.
This study evaluated the immune bioactivity of testing media (TM) obtained from different calcium silicate-based sealers and cements on monocyte morphology, activation, differentiation and cytokine secretion.
Blood-derived CD14 monocytes were isolated and cultured for 5 days with 25% TM from the following calcium silicate-based materials: TotalFill BC RRM Fast-Set Putty, Biodentine, TotalFill BC Sealer and BioRoot-Root-Canal-Sealer (RCS). A resin-based endodontic cement was used as a control. The expression of surface markers such as CD86, HLA-DR, CD16, CD309 and CD209, and cytokine secretion were analysed by flow cytometry. Data were analysed using the one-way repeated measures analysis of variance (anova) multiple comparison test and a Holm-Sidak multiple comparison post-hoc test (p < .05).
This comparative analysis revealed that monocytes co-cultured with calcium silicate-based materials showed a spindle-shaped morphology compared with the round shape observed in the control. Regarding activation markers, BioRoot-RCS and Biodentine significantly increased CD86 expression compared with the control sample, whereas no significant differences (p > .05) were observed in HLA-DR expression. In addition, no differences were observed among the differentiation markers. When the inflammatory cytokines were analysed, BioRoot-RCS increased the secretion of IL-1β, IL-6, IL-10 and TNF-α, whereas BioRoot-RCS and Biodentine significantly decreased IL-8 production (p < .05).
These data showed that the calcium silicate-based materials tested changed the morphology of CD14 monocytes; however, only BioRoot-RCS and Biodentine significantly upregulated CD86. In addition, BioRoot-RCS was the sealer with the highest immunomodulatory properties for cytokine production which means that it can contribute with the in vivo healing process and regeneration of periapical lesions.
本研究评估了从不同硅酸钙类封闭剂和水门汀获得的测试介质（TM）对单核细胞形态、活化、分化和细胞因子分泌的免疫生物活性。
分离血源性CD14单核细胞，并用来自以下硅酸钙类材料的25%TM培养5天：TotalFill BC RRM快速凝固糊剂、Biodentine、TotalFill BC封闭剂和BioRoot根管封闭剂（RCS）。使用树脂类牙髓水门汀作为对照。通过流式细胞术分析表面标志物如CD86、HLA-DR、CD16、CD309和CD209的表达以及细胞因子分泌。使用单因素重复测量方差分析（anova）多重比较检验和Holm-Sidak多重比较事后检验分析数据（p <.05）。
该比较分析显示，与对照中观察到的圆形形态相比，与硅酸钙类材料共培养的单核细胞呈现纺锤形形态。关于活化标志物，与对照样品相比，BioRoot-RCS和Biodentine显著增加了CD86表达，而在HLA-DR表达中未观察到显著差异（p >.05）。此外，在分化标志物之间未观察到差异。当分析炎性细胞因子时，BioRoot-RCS增加了IL-1β、IL-6、IL-10和TNF-α的分泌，而BioRoot-RCS和Biodentine显著降低了IL-8的产生（p <.05）。
这些数据表明，所测试的硅酸钙类材料改变了CD14单核细胞的形态；然而，只有BioRoot-RCS和Biodentine显著上调了CD86。此外，BioRoot-RCS是细胞因子产生免疫调节特性最高的封闭剂，这意味着它可以促进体内愈合过程和根尖周病变的再生。
