采用依赖/sites 结合模型对协同核糖开关进行等温滴定量热法分析。

Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model.

机构信息

Department of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.

Center for RNA Biology, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.

出版信息

Methods Mol Biol. 2023;2568:53-73. doi: 10.1007/978-1-0716-2687-0_5.

DOI:10.1007/978-1-0716-2687-0_5

PMID:36227562

Abstract

Isothermal titration calorimetry (ITC) is a powerful biophysical tool to characterize energetic profiles of biomacromolecular interactions without any alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch-ligand interaction.

摘要

等温滴定量热法（ITC）是一种强大的生物物理工具，可用于在不改变潜在化学结构的情况下，对生物大分子相互作用的能量分布进行特征描述。在本方案中，我们将描述从协同型核糖开关-配体相互作用中获得 ITC 数据的执行、分析和解释的程序。