College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China.
Shenzhen Institute of Guangdong Ocean University, Shenzhen, Guangdong, 518114, China; School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China.
Biochem Biophys Res Commun. 2022 Dec 17;634:10-19. doi: 10.1016/j.bbrc.2022.09.115. Epub 2022 Oct 4.
Extracellular vesicles (EVs) and their exosome subsets are vesicle-like nanoparticles (EVs) that are secreted by cells and contain various factors that treat various diseases. However, studies on extracting EVs from marine shellfish are still relatively lacking. In this study, EVs were isolated from Pinctada martensii mucus and the efficacy of EVs in modulating the inflammatory environment was demonstrated. A human skin inflammatory cell model was established to investigate the effect of Pinctada martensii mucus-derived EVs on inflammation. The results showed that EVs could restore the viability of inflammatory HaCaT cells and decrease the level of reactive oxygen species (ROS), as well as the mRNA expression of IL-6, IL-8 and TNF-α. The inflammation of HaCaT cells was treated by inhibiting the activation of the MAPK, NF-κB and NLRP3 inflammasome signaling pathways, which prevented the phosphorylation of related inflammatory proteins and the entry of P65 protein into the nucleus. This study provides novel EVs from marine shellfish-derived bioactive materials.
细胞外囊泡 (EVs) 及其外泌体亚群是由细胞分泌的囊泡样纳米颗粒 (EVs)，其中包含各种治疗各种疾病的因子。然而，关于从海洋贝类中提取 EVs 的研究仍然相对较少。在这项研究中，从马氏珠母贝粘液中分离出 EVs，并证明了 EVs 在调节炎症环境中的功效。建立了人皮肤炎症细胞模型，以研究马氏珠母贝粘液衍生的 EVs 对炎症的影响。结果表明，EVs 可以恢复炎症性 HaCaT 细胞的活力，降低活性氧 (ROS) 的水平，以及 IL-6、IL-8 和 TNF-α 的 mRNA 表达。通过抑制 MAPK、NF-κB 和 NLRP3 炎症小体信号通路的激活来治疗 HaCaT 细胞的炎症，从而阻止相关炎症蛋白的磷酸化和 P65 蛋白进入细胞核。本研究提供了来自海洋贝类的新型 EVs 衍生的生物活性物质。
