Translational Gerontology Branch, National Institute on Aging, National Institutes of Health, Baltimore, MD, 21224, USA.
Laboratory of Behavioral Neuroscience, National Institute on Aging, National Institutes of Health, Baltimore, MD, 21224, USA.
Sci Rep. 2022 Oct 13;12(1):17147. doi: 10.1038/s41598-022-22116-0.
SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.
SomaScan 是一种高通量、基于适体的蛋白质组学检测方法，旨在同时测量数千种具有广泛内源性浓度的蛋白质。在其最新版本中，7k SomaScan 检测 v4.1 能够测量 7288 个人类蛋白质。在这项工作中，我们基于对 22 个板上 2050 个样本的研究，对该平台进行了广泛的技术评估。研究设计中包括来自 102 个人类受试者的板间技术重复，这使我们能够描述不同的标准化程序，通过多种分析方法评估检测的可变性，提供信号背景比的指标，并讨论潜在的特异性问题。通过对这些广泛的技术方面进行详细的性能评估，我们希望这项工作能够成为不断发展的 SomaScan 用户群体的宝贵资源。
