Chen Jiangang, Xu Xinyun, Dalhaimer Paul, Zhao Ling
Department of Public Health, The University of Tennessee, Knoxville, TN 37996, USA.
Department of Nutrition, The University of Tennessee, Knoxville, TN 37996, USA.
Animals (Basel). 2022 Oct 5;12(19):2680. doi: 10.3390/ani12192680.
Due to spontaneous deficiency in leptin, / mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping method using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) to differentiate mice with a mutated allele from the wild-type genotype. The amplicon patterns of different genotypes are clearly visible and distinguishable on 1.5% agarose gel. This method can serve as a valuable tool to differentiate genotypes for breeding purposes, to maintain animal colonies, control the available space in the animal facility, and identify appropriate individuals for animal experiments.
由于瘦素的自发缺陷，/小鼠是糖尿病研究中最常用的实验动物模型之一。在本研究中，我们报道了一种快速且易于实施的基因分型方法，即使用四引物扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）来区分具有突变等位基因的小鼠与野生型基因型。不同基因型的扩增产物模式在1.5%琼脂糖凝胶上清晰可见且易于区分。该方法可作为一种有价值的工具，用于区分基因型以进行育种、维持动物种群、控制动物设施中的可用空间以及识别适合动物实验的个体。
