Xu Xinyun, Hu Xinge, Ma Guodong, Wang Tiannan, Wu Jayne, Zhu Xiaojuan, Chen Guoxun, Zhao Ling, Chen Jiangang
Department of Nutrition, The University of Tennessee Knoxville, TN, 37996, United States.
Department of Electrical Engineering and Computer Science, The University of Tennessee Knoxville, TN, 37996, United States.
Heliyon. 2023 Sep 14;9(9):e20159. doi: 10.1016/j.heliyon.2023.e20159. eCollection 2023 Sep.
Due to the genetic mutation () in the gene encoding for leptin receptor, homozygous Zucker rats () develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), we developed a method to quickly genotype Zucker rats with a mutated allele from their wildtype littermates. The three genotypes are clearly discriminated on 2.0% agarose gel. Our method can be used as a reliable tool to set up and maintain the breeding colony in animal facilities as well as assign animals to control and treatment groups based on their genotypes for animal studies.
由于瘦素受体编码基因中的基因突变（），纯合子 Zucker 大鼠（）会出现过度肥胖，成为肥胖和代谢相关疾病研究中的实验动物模型。基于四引物扩增阻滞突变系统-聚合酶链反应（ARMS-PCR），我们开发了一种方法，能够快速从野生型同窝仔鼠中对携带突变等位基因的 Zucker 大鼠进行基因分型。在 2.0%的琼脂糖凝胶上可以清晰区分这三种基因型。我们的方法可作为一种可靠工具，用于在动物设施中建立和维持繁殖群体，以及根据动物基因型将其分配到对照组和治疗组以进行动物研究。
