Military Therapy Department, Yerevan State Medical University, Yerevan 0025, Armenia.
The International Scientific-Educational Center of NAS RA, Yerevan 0019, Armenia.
Cells. 2022 Sep 26;11(19):2989. doi: 10.3390/cells11192989.
Either extracts, cell-free suspensions or bacterial suspensions are used to study bacterial lipid peroxidation processes. Along with gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and several other strategies, the thiobarbituric acid test is used for the determination of malondialdehyde (MDA) as the basis for the commercial test kits and the colorimetric detection of lipid peroxidation. The aim of the current study was to evaluate lipid peroxidation processes levels in the suspensions, extracts and culture supernatants of and Derby strains. The dependence of the formation of thiobarbituric acid-reactive substances levels in the cell extracts, the suspensions and cell-free supernatants on bacterial species, and their concentration and growth phase were revealed. The effect of bacterial concentrations on MDA formation was also found to be more pronounced in bacterial suspensions than in extracts, probably due to the dynamics of MDA release into the intercellular space. This study highlights the possible importance of MDA determination in both cell-free suspensions and extracts, as well as in bacterial suspensions to elucidate the role of lipid peroxidation processes in bacterial physiology, bacteria-host interactions, as well as in host physiology.
无论是提取物、无细胞悬浮液还是细菌悬浮液，都可用于研究细菌脂质过氧化过程。除了气相色谱-质谱法、液相色谱-质谱法和其他几种策略外，还使用硫代巴比妥酸试验来测定丙二醛（MDA），作为商业试剂盒的基础和脂质过氧化的比色检测。本研究旨在评估 和 Derby 菌株的悬浮液、提取物和培养上清液中的脂质过氧化过程水平。揭示了细胞提取物、悬浮液和无细胞上清液中硫代巴比妥酸反应物质水平的形成与细菌种类及其浓度和生长阶段的依赖性。还发现，与提取物相比，细菌浓度对 MDA 形成的影响在细菌悬浮液中更为明显，这可能是由于 MDA 向细胞间隙释放的动力学不同所致。本研究强调了在无细胞悬浮液和提取物以及细菌悬浮液中测定 MDA 的重要性，以阐明脂质过氧化过程在细菌生理学、细菌-宿主相互作用以及宿主生理学中的作用。
