Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing 210014, China.
Int J Mol Sci. 2022 Oct 8;23(19):11967. doi: 10.3390/ijms231911967.
Members of the N-rich proteins (NRPs) gene family play important roles in the plant endoplasmic reticulum stress in response, which can be triggered by plant pathogens' infection. Previous studies of the gene family have been limited to only a few plants, such as soybean and . Thus, their evolutionary characteristics in the species and biological functions in rice defense against the pathogenic fungus have remained unexplored. In the present study, we demonstrated that the genes family may have originated in the early stages of plant evolution, and that they have been strongly conserved during the evolution of the species. Domain organization of NRPs was found to be highly conserved within but not between subgroups. , an gene in the group, was specifically up-regulated during the early stages of rice- interactions-inhibited infection. Predicted protein-protein interaction networks and transcription-factor binding sites revealed a candidate interactor, bZIP50, which may be involved in mediated rice resistance against infection. Taken together, our results established a basis for future studies of the gene family and provided molecular insights into rice immune responses to .
富含氮的蛋白质（NRPs）基因家族的成员在植物内质网应激反应中发挥着重要作用，这种反应可能是由植物病原体的感染引发的。以前对该基因家族的研究仅限于少数几种植物，如大豆和。因此，它们在物种中的进化特征以及在水稻抵御致病真菌的防御中的生物学功能仍未得到探索。在本研究中，我们证明了 基因家族可能起源于植物进化的早期阶段，并且在 物种的进化过程中得到了强烈的保守。NRPs 的结构域组织在亚群内高度保守，但在亚群之间不保守。属于 亚群的 基因在水稻与 互作抑制感染的早期阶段特异性地上调。预测的蛋白质-蛋白质相互作用网络和转录因子结合位点揭示了一个候选的相互作用因子 bZIP50，它可能参与介导水稻对 感染的抗性。总之，我们的研究结果为进一步研究 基因家族奠定了基础，并为水稻对 感染的免疫反应提供了分子见解。
