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靶向转录因子通过与 （）启动子结合来调控开花时间。

The -Targeted () Transcription Factor Regulates the Flowering Time by Binding to the Promoter of () in .

机构信息

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of the Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.

出版信息

Int J Mol Sci. 2022 Oct 9;23(19):11976. doi: 10.3390/ijms231911976.

DOI:10.3390/ijms231911976
PMID:36233277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9570364/
Abstract

, a famous perennial ornamental plant and fruit tree in Asia, blooms in winter or early spring in the Yangtze River area. The flowering time directly determines its ornamental and economic value, so it is of great significance to study the molecular mechanism of flowering time. (), often regulated by , is an important flowering regulator, although its function is unknown in . Here, 11 precursors were analyzed and located in five chromosomes of the genome. The expression pattern showed that / was negatively correlated with . The promoters of / were specifically expressed in the apical meristem. Overexpression of / in tobacco promoted flowering and changed the length ratio of pistil and stamen. Moreover, also affected the number and vitality of pollen and reduced the fertility of transgenic tobacco. Furthermore, ectopic expression of caused up-regulated expression of endogenous (). The yeast-one hybrid assay showed that PmSBP1 was bonded to the promoters of . In conclusion, a miR156-PmSBP1-PmSOC1s pathway was formed to participate in the regulation of flowering time in , which provided references for the molecular mechanism of flowering time regulation and molecular breeding of

摘要

桃，亚洲著名的多年生观赏植物和果树，在长江流域冬季或早春开花。开花时间直接决定了其观赏和经济价值，因此研究开花时间的分子机制具有重要意义。（），通常受（）调控，是一种重要的开花调节剂，尽管其功能在（）中尚不清楚。在这里，分析了 11 个前体，并定位在（）基因组的五个染色体上。表达模式表明，/与（）呈负相关。/的启动子在顶端分生组织中特异性表达。过量表达烟草中的/会促进开花，并改变雌蕊和雄蕊的长度比。此外，还会影响花粉的数量和活力，降低转基因烟草的育性。此外，异位表达（）导致内源（）的表达上调（）。酵母单杂交试验表明，PmSBP1 与（）的启动子结合。总之，形成了一个 miR156-PmSBP1-PmSOC1s 途径，参与了（）开花时间的调控，为开花时间调控的分子机制和分子育种提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/d32acf579f7b/ijms-23-11976-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/d0cf0c6bb2dc/ijms-23-11976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/6826bacabe6e/ijms-23-11976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/4b1296c29a2c/ijms-23-11976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/aaf8bacc02cf/ijms-23-11976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/25855048e2d7/ijms-23-11976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/7bfc2d65c617/ijms-23-11976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/3ff4bd307711/ijms-23-11976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/52da4bd495e7/ijms-23-11976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/d32acf579f7b/ijms-23-11976-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/d0cf0c6bb2dc/ijms-23-11976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/6826bacabe6e/ijms-23-11976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/4b1296c29a2c/ijms-23-11976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/aaf8bacc02cf/ijms-23-11976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/25855048e2d7/ijms-23-11976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/7bfc2d65c617/ijms-23-11976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/3ff4bd307711/ijms-23-11976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/52da4bd495e7/ijms-23-11976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/690f/9570364/d32acf579f7b/ijms-23-11976-g009.jpg

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