Morgan R L, Sorenson S S, Castles T R
Food Chem Toxicol. 1987 Aug;25(8):609-13. doi: 10.1016/0278-6915(87)90022-6.
Corneal pachymetry performed 3 days after application of a variety of test materials to the rabbit eye was found to be predictive of the eye irritation classification determined by observing the ocular response for 21 days. The test materials included NaOH, methanol, isopropanol, acetone, cyclohexanone, hexane and a shampoo. A 0.1-ml sample of the test material was placed in the conjunctival sac in the left eye of each rabbit. Both the left and the right eye of each rabbit were evaluated for irritation and corneal thickness for up to 21 days using a slit-lamp biomicroscope with a pachymeter attachment. On day 3 of observation the mean corneal thickness ratios (treated/control eye) were predictive of the duration of corneal cloudiness (correlation coefficient = 0.86). In addition, these ratios were predictive of the eye irritation classification as determined by a 21-day test (correlation coefficient = 0.98). Corneal pachymetry for determining eye irritation classification is presented as an alternative to the current 21-day test. It is more objective and requires a shorter observation period. Therefore, this method should lessen the cost of eye irritation testing and decrease the duration of discomfort that may occur among the test animals. The greater objectivity may also reduce the intra- and interlaboratory variation and the number of rabbits required for each testing.
在兔眼应用多种测试材料3天后进行角膜测厚，发现其可预测通过观察21天眼部反应所确定的眼刺激分类。测试材料包括氢氧化钠、甲醇、异丙醇、丙酮、环己酮、己烷和一种洗发水。将0.1毫升测试材料样品置于每只兔子左眼的结膜囊中。使用带有测厚仪附件的裂隙灯生物显微镜，对每只兔子的左眼和右眼进行长达21天的刺激和角膜厚度评估。在观察的第3天，平均角膜厚度比（处理眼/对照眼）可预测角膜混浊的持续时间（相关系数 = 0.86）。此外，这些比值可预测通过21天测试所确定的眼刺激分类（相关系数 = 0.98）。提出用角膜测厚来确定眼刺激分类，作为当前21天测试的替代方法。它更客观，且观察期更短。因此，这种方法应能降低眼刺激测试的成本，并减少试验动物可能出现的不适持续时间。更高的客观性还可能减少实验室内部和实验室之间的差异以及每次测试所需兔子的数量。
