Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad 500 085, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India.
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1790-1798. doi: 10.1099/ijs.0.026815-0. Epub 2011 Oct 10.
Four strains (JA310(T), JA531(T), JA447 and JA490) of red to reddish brown pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from soil and freshwater sediment samples from different geographical regions of India. All strains contained bacteriochlorophyll a and carotenoids of the spirilloxanthin series. The major cellular fatty acid of strains JA310(T) and JA531(T) was C(18:1)ω7c, the quinone was Q-10 and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminohopanoid and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that all strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria. Strains JA531(T), JA447 and JA490 were genotypically (>80% related based on DNA-DNA hybridization) and phenotypically closely related to each other and the three strains were distinct from strain JA310(T) (33% related). Furthermore, all four strains had less than 48% relatedness (DNA-DNA hybridization) with type strains of members of the genus Rhodopseudomonas, i.e. Rhodopseudomonas palustris ATCC 17001(T), Rhodopseudomonas faecalis JCM 11668(T) and Rhodopseudomonas rhenobacensis DSM 12706(T). The genomic DNA G+C contents of strains JA310(T) and JA531(T) were 63.8 and 62.4 mol%, respectively. On the basis of phenotypic, chemotaxonomic and molecular genetic evidence, it is proposed that strains JA310(T) ( = NBRC 106083(T) = KCTC 5839(T)) and JA531(T) ( = NBRC 107575(T) = KCTC 5841(T)) be classified as the type strains of two novel species of the genus Rhodopseudomonas, Rhodopseudomonas parapalustris sp. nov. and Rhodopseudomonas harwoodiae sp. nov., respectively. In addition, we propose that strain DSM 123(T) ( = NBRC 100419(T)) represents a novel species, Rhodopseudomonas pseudopalustris sp. nov., since this strain differs genotypically and phenotypically from R. palustris ATCC 17001(T) and other members of the genus Rhodopseudomonas. An emended description of R. palustris is also provided.
从印度不同地理区域的土壤和淡水沉积物样本中分离到四株（JA310(T)、JA531(T)、JA447 和 JA490）红色到红棕色色素、杆状、运动和出芽的光养细菌。所有菌株均含有菌叶绿素 a 和螺旋藻叶黄素系列的类胡萝卜素。菌株 JA310(T)和 JA531(T)的主要细胞脂肪酸为 C(18:1)ω7c，醌为 Q-10，极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种氨基胡醇和一种未鉴定的氨基脂。基于 16S rRNA 基因序列的系统发育分析表明，所有菌株均与 α-变形菌纲的红假单胞菌属的物种聚在一起。菌株 JA531(T)、JA447 和 JA490 在基因型（基于 DNA-DNA 杂交的 >80%相关）和表型上彼此密切相关，并且这三个菌株与菌株 JA310(T)（33%相关）明显不同。此外，所有四个菌株与红假单胞菌属成员的模式株的相关性小于 48%（DNA-DNA 杂交），即沼泽红假单胞菌 ATCC 17001(T)、粪产碱假单胞菌 JCM 11668(T)和莱茵河假单胞菌 DSM 12706(T)。菌株 JA310(T)和 JA531(T)的基因组 DNA G+C 含量分别为 63.8 和 62.4 mol%。基于表型、化学分类学和分子遗传学证据，建议菌株 JA310(T)( = NBRC 106083(T) = KCTC 5839(T))和 JA531(T)( = NBRC 107575(T) = KCTC 5841(T))分别被分类为红假单胞菌属的两个新种的模式株，即拟沼泽红假单胞菌 sp. nov.和 Harwoodiae 红假单胞菌 sp. nov.。此外，我们建议菌株 DSM 123(T)( = NBRC 100419(T))代表一个新种，即沼泽红假单胞菌 pseudopalustris sp. nov.，因为该菌株在基因型和表型上与沼泽红假单胞菌 ATCC 17001(T)和其他红假单胞菌属成员不同。还提供了对沼泽红假单胞菌的修订描述。
