Hiraishi Akira, Okamura Keiko
Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
Electronics- Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3369-3374. doi: 10.1099/ijsem.0.002120. Epub 2017 Sep 6.
A strain of anoxygenic phototrophic bacteria isolated from paddy soil (designated strain TUT3615T) was studied taxonomically in comparison with Rhodopseudomonasstrain ATCC 17005 as its nearest phylogenetic relative. Strains TUT3615T and ATCC 17005 had budding rod-shaped cells and showed in vivo absorption maxima at 804 and 860 nm in the near infrared region, indicating the presence of bacteriochlorophyll a. The intracytoplasmic membrane system was of the lamellar type parallel to the cytoplasmic membrane. 16S rRNA gene sequence comparisons showed that strains TUT3615T and ATCC 17005 had a 99.7 % level of similarity to one another and were closest to Rhodopseudomonas palustris ATCC 17001T (98.6 % similarity) among the established species of the genus Rhodopseudomonas. Genomic DNA-DNA hybridization studies revealed that strains TUT3615T and ATCC 17005 had an average similarity level of 65 % to one another and of less than 40 % to the available type strains of Rhodopseudomonas species. Results of phenotypic studies showed that strains TUT3615T and ATCC 17005 could be differentiated from one another and from any previously described species of Rhodopseudomonas. The G+C contents of the genomic DNA of strain TUT3615T and ATCC 17005 were 66.3 and 66.5 mol%, respectively. Based on these data, we propose the name Rhodopseudomonas telluris sp. nov. for strain TUT3615T. The type strain is TUT3615T (=KCTC 23279T=NBRC 107609T). We suspend a proposal to reclassify strain ATCC 17005 as a novel species or subspecies until a genome-wide analysis provides more definite information on its taxonomic position.
从稻田土壤中分离出的一株无氧光合细菌（命名为菌株TUT3615T），与最接近的系统发育相关菌株红假单胞菌菌株ATCC 17005进行了分类学研究。菌株TUT3615T和ATCC 17005具有出芽杆状细胞，在近红外区域的体内吸收最大值分别为804和860 nm，表明存在细菌叶绿素a。胞内膜系统为与细胞质膜平行的层状类型。16S rRNA基因序列比较表明，菌株TUT3615T和ATCC 17005彼此间的相似性水平为99.7%，在红假单胞菌属已确立的物种中，它们与沼泽红假单胞菌ATCC 17001T最为接近（相似性为98.6%）。基因组DNA-DNA杂交研究表明，菌株TUT3615T和ATCC 17005彼此间的平均相似性水平为65%，与红假单胞菌属现有模式菌株的相似性低于40%。表型研究结果表明，菌株TUT3615T和ATCC 17005可以相互区分，也可以与任何先前描述的红假单胞菌物种区分开来。菌株TUT3615T和ATCC 17005的基因组DNA的G+C含量分别为66.3和66.5 mol%。基于这些数据，我们提议将菌株TUT3615T命名为陆地红假单胞菌（Rhodopseudomonas telluris sp. nov.）。模式菌株为TUT3615T（=KCTC 23279T=NBRC 107609T）。在全基因组分析提供有关其分类地位的更明确信息之前，我们暂不提议将菌株ATCC 17005重新分类为新物种或亚种。
