Cortés-Patiño Sandra, Vargas Christian D, Alvarez-Flórez Fagua, Estrada-Bonilla German
Rothamsted Research, Protection of Crops and the Environment, Harpenden, Hertfordshire AL5 2JQ, UK.
School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK.
Plants (Basel). 2022 Sep 28;11(19):2543. doi: 10.3390/plants11192543.
Perennial ryegrass is a forage commonly used in temperate regions for livestock feeding; however, its yield is affected by reduced biomass production under water deficit. In a previous study, three co-inoculations of beneficial bacteria were selected based on their ability to promote plant growth under reduced water availability. The aim of this work was to elucidate some mechanisms by which the selected bacteria can help improve the response of perennial ryegrass to water deficit. Ryegrass plants were inoculated with each of the co-inoculations (Herbaspirillum sp. AP02−Herbaspirillum sp. AP21; Herbaspirillum sp. AP02−Pseudomonas sp. N7; Herbaspirillum sp. AP21−Azospirillum brasilense D7) and subjected to water deficit for 10 days. Physiological and biochemical measurements were taken 10 days after stress and shortly after rehydration. The results showed that bacteria had a positive effect on shoot biomass production, dissipation of excess energy, and proline and chlorophyll pigments during the days of water deficit (p < 0.05). The leaf water status of the inoculated plants was 12% higher than that of the uninoculated control after rehydration. Two Herbaspirillum strains showed greater potential for use as biofertilizers that help ameliorate the effects of water deficit.
多年生黑麦草是温带地区常用的一种用于饲养牲畜的牧草；然而，在水分亏缺条件下，其生物量生产减少，从而影响产量。在之前的一项研究中，根据有益细菌在水分供应减少的情况下促进植物生长的能力，选择了三种联合接种组合。这项工作的目的是阐明所选细菌有助于改善多年生黑麦草对水分亏缺响应的一些机制。黑麦草植株分别接种每种联合接种组合（固氮螺菌属AP02菌株 - 固氮螺菌属AP21菌株；固氮螺菌属AP02菌株 - 假单胞菌属N7菌株；固氮螺菌属AP21菌株 - 巴西固氮螺菌D7菌株），并遭受10天的水分亏缺处理。在胁迫10天后和复水后不久进行生理和生化测量。结果表明，在水分亏缺期间，细菌对地上部生物量生产、过剩能量的耗散以及脯氨酸和叶绿素色素有积极影响（p < 0.05）。复水后，接种植株的叶片水分状况比未接种对照高12%。两种固氮螺菌菌株显示出作为生物肥料的更大潜力，有助于减轻水分亏缺的影响。
