Vilkickyte Gabriele, Zilius Modestas, Petrikaite Vilma, Raudone Lina
Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania.
Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania.
Plants (Basel). 2022 Oct 5;11(19):2615. doi: 10.3390/plants11192615.
The compositions and health-beneficial properties of lingonberry leaves ( L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and 'wound-healing' properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A 'wound healing' in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate 'wound' closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations.
越橘叶（Vaccinium vitis-idaea L.）的成分及其有益健康的特性已得到充分证实；然而，其原花青素仍未得到充分利用。优化其递送系统是实现其更广泛应用的关键。本研究调查了越橘叶富含原花青素组分（PRF）的植物化学和“伤口愈合”特性，以及作为原花青素递送系统的最佳真皮膜的开发。对获得的PRF进行HPLC-PDA和DMAC分析，以确认不同聚合度原花青素的定性和定量概况。进行了“伤口愈合”体外试验，以评估PRF调节伤口环境以促进更好愈合的能力。发现低浓度的越橘原花青素可加速“伤口”闭合，而高浓度则抑制人类成纤维细胞迁移。制备了15种含有PRF的真皮膜，并根据其聚合物（MC、HEC、PEG 400）组成以及物理、机械和生物药剂学性质，采用实验设计进行评估。含有0.30 g MC、0.05 g HEC和3.0 g PEG 400的组合物被选为PRF递送的有前景配方和潜在有效的功能性伤口敷料材料，这支持了进一步研究的必要性。
