Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162, Kaunas, Lithuania.
Laboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162, Kaunas, Lithuania.
J Ethnopharmacol. 2022 Jun 28;292:115207. doi: 10.1016/j.jep.2022.115207. Epub 2022 Mar 17.
Vaccinium vitis-idaea L. (lingonberry) leaves and fruits have traditionally been used in Asian and European countries as a natural solution for urinary tract infections, gastrointestinal distress, neurodegenerative diseases, and related inflammatory disorders, which are overall associated with free radical damage and presence of triggering pathogenic strains in the human body. Considering growing attention to natural products, there are not enough scientific data to confirm predominant specialized metabolites, responsible for the traditional therapeutic use of lingonberries.
The present study aimed at an in-depth study of specialized metabolite profiling and biological activity evaluation of lingonberry crude extracts and isolated fractions.
Crude dry extracts and fractions from lingonberry leaves and fruits were analyzed by the UPLC-MS method. Potential inhibiting properties against different bacterial strains and hyaluronidase, ability to scavenge hydrogen peroxide, and effect on its production in a macrophage culture J774 were examined.
Findings suggested the tentative presence of 59 compounds, mainly phenolics, displayed higher bioactivities of particular fractions than that of crude extracts and elucidated particular compounds as candidates in pharmaceuticals. Trimeric and dimeric proanthocyanidins from lingonberry leaves and fruits were shown to have the strongest antimicrobial, antioxidant, and anti-inflammatory potential.
This study revealed specialized metabolites responsible for the traditional medicinal properties of lingonberries and pointed out demand for further purification and new research directions of proanthocyanidins in the frame of their multipharmacological perspectives.
越桔（蔓越莓）的叶子和果实传统上被亚洲和欧洲国家用作治疗尿路感染、胃肠道不适、神经退行性疾病和相关炎症性疾病的天然方法，这些疾病总体上与自由基损伤和人体中触发致病菌株的存在有关。考虑到对天然产物的关注度不断增加，目前还没有足够的科学数据来证实负责蔓越莓传统治疗用途的主要特殊代谢物。
本研究旨在深入研究蔓越莓粗提取物和分离馏分的特殊代谢产物谱和生物活性。
采用 UPLC-MS 法分析越桔叶和果实的粗干提取物和馏分。检测了它们对不同细菌菌株和透明质酸酶的抑制特性、清除过氧化氢的能力以及对巨噬细胞 J774 培养物中过氧化氢产生的影响。
研究结果表明，存在 59 种化合物，主要为酚类化合物，某些馏分的生物活性高于粗提取物，并阐明了某些化合物可能是药物中的候选物。越桔叶和果实中的三聚体和二聚体原花青素表现出最强的抗菌、抗氧化和抗炎潜力。
本研究揭示了负责蔓越莓传统药用特性的特殊代谢物，并指出需要进一步纯化，并从多药理学角度研究原花青素的新研究方向。
