Somade Oluwatobi T, Oyinloye Babatunji E, Ajiboye Bashiru O, Osukoya Olukemi A, Adeyi Olubisi E
Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria.
Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti, 360001, Nigeria.
Biochem Biophys Rep. 2022 Oct 7;32:101360. doi: 10.1016/j.bbrep.2022.101360. eCollection 2022 Dec.
Methyl cellosolve (MTC) is an established gonadotoxic and hematotoxic compound that is commonly and universally utilized in herbicide, liquid soap, stain, dye, paint, and brake fluid manufacturing industries as a solvent. Due to its wide range usage, this study therefore investigated the effect of syringic acid (SYAC) on hematological indices, sperm characteristics and morphologies, and markers of tissue damage in MTC administered male Wistar rats. Thirty (30) rats divided into six groups were used. Rats in group 1 served as control, those in group 2 were administered MTC for 30 consecutive days, those in groups 3, 4, and 5 were treated with 25, 50, and 75 mg/kg body weight of SYAC respectively also for 30 consecutive days immediately after each day MTC administrations, while rats in group 6 received 75 mg/kg body weight of SYAC only throughout. Compared with control, administrations of MTC resulted in a significant decrease in spermatozoa count, number of normal and live spermatozoa, Hb count, MCH, MCHC, serum TC, and LH, while number of abnormal spermatozoa, RBC and WBC counts, activities of serum AST, ALT, GGT, LDH, and ADH were significantly increased. Treatments with 25 mg/kg of SYAC significantly reduced the RBC and WBC counts, serum activities of AST, ALT, GGT, and increased TC concentration. Treatments with 50 mg/kg SYAC significantly lowered the number of abnormal spermatozoa, RBC count, activities of serum ALT, AST, LDH, ADH, and increased the number of normal spermatozoa, MCV, MCH, and MCHC, while 75 mg/kg of SYAC significantly decreased the serum activities of AST, ALT, GGT, LDH, ADH, and increased serum TC concentration. Findings from this study have revealed the hepatoprotective effect of SYAC at all doses investigated but did not confer spermatoprotection and hematoprotection against MTC-induced toxicities, and looking at the 3 doses investigated, 50 mg/kg of SYAC yielded the best effect.
甲基溶纤剂（MTC）是一种公认的具有性腺毒性和血液毒性的化合物，在除草剂、液体肥皂、污渍清除剂、染料、油漆和制动液制造行业中普遍用作溶剂。由于其广泛的用途，本研究因此调查了丁香酸（SYAC）对经MTC处理的雄性Wistar大鼠血液学指标、精子特征和形态以及组织损伤标志物的影响。使用了30只分为六组的大鼠。第1组大鼠作为对照，第2组大鼠连续30天给予MTC，第3、4和5组大鼠在每天给予MTC后，也分别连续30天给予25、50和75mg/kg体重的SYAC，而第6组大鼠仅全程给予75mg/kg体重的SYAC。与对照组相比，给予MTC导致精子计数、正常和活精子数量、血红蛋白计数、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）、血清总胆固醇（TC）和促黄体生成素（LH）显著降低，而异常精子数量、红细胞和白细胞计数、血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、γ-谷氨酰转移酶（GGT）、乳酸脱氢酶（LDH）和乙醇脱氢酶（ADH）活性显著增加。用25mg/kg的SYAC处理显著降低了红细胞和白细胞计数、血清AST、ALT、GGT活性，并增加了TC浓度。用50mg/kg SYAC处理显著降低了异常精子数量、红细胞计数、血清ALT、AST、LDH、ADH活性，并增加了正常精子数量、平均红细胞体积（MCV）、MCH和MCHC，而75mg/kg的SYAC显著降低了血清AST、ALT、GGT、LDH、ADH活性，并增加了血清TC浓度。本研究结果表明，在所研究的所有剂量下，SYAC均具有肝脏保护作用，但对MTC诱导的毒性没有精子保护和血液保护作用，在所研究的3个剂量中，50mg/kg的SYAC效果最佳。
