Department of Gynecology, Wuhan Childrens Hospital, Wuhan Maternal and Child Health Hospital, Wuhan Womens and Childrens Health Care Center, Wuhan, Hubei Province, China.
Contrast Media Mol Imaging. 2022 Sep 26;2022:8497078. doi: 10.1155/2022/8497078. eCollection 2022.
To investigate the effect of SRSF3 on the viability and metastasis of cervical cancer (CC) SiHa and Hela cells.
In vitro, HeLa cells and SiHa cells were cultured. In cervical cancer cells, RNA interference technology was utilized to lessen the SRSF3 level, and via RT-PCR utilization, the SRSF3 level in every group of cells was revealed. By employing the CCK-8 method, the OD value was revealed in every group at 24, 48, 72, and 96 h. On the migration of cervical cancer SiHa and HeLa cells via transwell utilizing, the consequence of SRSF3 was surveyed. Through western blotting utilizing, the PI3K/AKT/mTOR signaling pathway-connected proteins levels was revealed.
In SiHa cells, contrasted to the NC-SiHa group, the SRSF3 level, the number of invasive cells per unit area, the p-PI3K/PI3K level, the p-AKT/AKT level, and the p-mTOR/mTOR level in the si-SRSF3 group were substantially lessened. The OD value at 490 nm of the si-SRSF3 group had no impressive divergence, contrasted to the NC-SiHa group at 24 h. At 48 h, the OD value of the si-SRSF3 group was impressively lessened than that of the NC-SiHa group. This connection was time-dependent. In HeLa cells, the SRSF3 level, the number of invasive cells per unit area, the level of p-PI3K/PI3K, the level of p-AKT/AKT, and the level of p-mTOR/mTOR in the cells of the si-SRSF3 group in the NC-HeLa group were impressively lessened than those in the NC-Hela group. Between the NC-HeLa group and the si-SRSF3 group at 24 h, there was no impressive divergence in the OD value at 490 nm. At 48 h, the OD value of the si-SRSF3 group was impressively lessened than that of the NC-SiHa group. This connection is time-dependent.
Reducing the SRSF3 level can restrain the viability and metastasis of cervical cancer cells via restraining the PI3K/AKT/mTOR signaling pathway.
研究 SRSF3 对宫颈癌（CC）SiHa 和 Hela 细胞活力和转移的影响。
体外培养 HeLa 细胞和 SiHa 细胞。在宫颈癌细胞中，采用 RNA 干扰技术降低 SRSF3 水平，通过 RT-PCR 利用各组细胞的 SRSF3 水平。采用 CCK-8 法，在 24、48、72 和 96 h 时显示每组的 OD 值。通过 Transwell 观察 SRSF3 对宫颈癌细胞 SiHa 和 HeLa 迁移的影响。通过 Western blot 揭示 PI3K/AKT/mTOR 信号通路相关蛋白水平。
在 SiHa 细胞中，与 NC-SiHa 组相比，si-SRSF3 组的 SRSF3 水平、单位面积侵袭细胞数、p-PI3K/PI3K 水平、p-AKT/AKT 水平和 p-mTOR/mTOR 水平均显著降低。与 NC-SiHa 组相比，si-SRSF3 组在 24 h 时 490nm 处的 OD 值无明显差异。在 48 h 时，si-SRSF3 组的 OD 值明显低于 NC-SiHa 组。这种关系是时间依赖性的。在 HeLa 细胞中，与 NC-HeLa 组相比，si-SRSF3 组的 SRSF3 水平、单位面积侵袭细胞数、p-PI3K/PI3K 水平、p-AKT/AKT 水平和 p-mTOR/mTOR 水平均显著降低。与 NC-HeLa 组相比，24 h 时两组之间 490nm 处的 OD 值无明显差异。在 48 h 时，si-SRSF3 组的 OD 值明显低于 NC-SiHa 组。这种关系是时间依赖性的。
降低 SRSF3 水平可通过抑制 PI3K/AKT/mTOR 信号通路抑制宫颈癌的活力和转移。
Zotero 插件
