Cao Hui, Pan Guihua, Tang Shiqiang, Zhong Ni, Liu Huake, Zhou Haizhi, Peng Qin, Zou Yongbin
Department of Oncology, Chenzhou First People's Hospital, Chenzhou, People's Republic of China.
Onco Targets Ther. 2020 Mar 20;13:2369-2376. doi: 10.2147/OTT.S241366. eCollection 2020.
Cervical carcinoma (CC) is a serious threat to women's health and few effective therapeutic methods have been discovered. The purpose of this study is to explore the underlying mechanism of miR-145-5p in CC.
Bioinformatics methods were employed to analyze the gene expression data of CC from TCGA database. qRT-PCR was used to detect the expression of miR-145-5p and KLF5 in CC cells, and Western blot was employed for the examination of KLF5 protein level. The targeted relationship between miR-145-5p and KLF5 was verified by a dual-luciferase reporter assay. Moreover, CCK-8, wound healing assay and transwell invasion assay were used to analyze the effects of miR-145-5p overexpression or KLF5 silencing on the proliferation, migration and invasion of CC cells.
miR-145-5p was shown to be down-regulated in CC tissues and cells, while KLF5 was up-regulated. miR-145-5p could bind to the complementary sequence within the wild type KLF5 3'UTR rather than the mutant one. In addition, miR-145-5p could effectively down-regulate KLF5, in turn inhibiting the proliferation, migration and invasion of CC cells.
miR-145-5p regulates the proliferation, migration and invasion of CC cells by targeting KLF5.
宫颈癌（CC）对女性健康构成严重威胁，且尚未发现有效的治疗方法。本研究旨在探讨miR-145-5p在CC中的潜在作用机制。
运用生物信息学方法分析来自TCGA数据库的CC基因表达数据。采用qRT-PCR检测CC细胞中miR-145-5p和KLF5的表达，并用蛋白质免疫印迹法检测KLF5蛋白水平。通过双荧光素酶报告基因实验验证miR-145-5p与KLF5的靶向关系。此外，使用CCK-8、伤口愈合实验和Transwell侵袭实验分析miR-145-5p过表达或KLF5沉默对CC细胞增殖、迁移和侵袭的影响。
miR-145-5p在CC组织和细胞中表达下调，而KLF5表达上调。miR-145-5p可与野生型KLF5 3'UTR中的互补序列结合，而非突变型。此外，miR-145-5p可有效下调KLF5，进而抑制CC细胞的增殖、迁移和侵袭。
miR-145-5p通过靶向KLF5调节CC细胞的增殖、迁移和侵袭。
