Chung Moonyoung, Park Young Seok
Department of Neurosurgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University, Korea.
Department of Neurosurgery, Chungbuk National University Hospital, Cheongju, Korea.
J Korean Neurosurg Soc. 2023 Mar;66(2):121-132. doi: 10.3340/jkns.2022.0106. Epub 2022 Oct 13.
The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in an animal model.
Fourteen rats were underwent stereotactic operation, in which they were injected with an adeno-associated virus with channelrhodopsin (AAV2-hSyn-ChR2-mCherry) to the lateral one third of the PF. Behavior test was performed with and without optical stimulation 14 days after the injection of the virus. AIM of rat was examined using AIM score. After the behavior test, rat's brain was carefully extracted and the section was examined using a fluorescence microscope to confirm transfection of the PF.
Of the 14 rats, seven rats displayed evident involuntary abnormal movements. AIM scores were increased significantly after the stimulation compared to those at baseline. In rats with AIMs, mCherry expression was prominent in the PF, while the rats without AIM lacked with the mCherry expression.
AIMs could be reversibly induced by stimulating the PF through an optogenetic method.
束旁核（PF）在控制基底神经节方面发挥着重要作用。目前尚不清楚PF是否会影响异常不自主运动（AIMs）的发展。本研究旨在通过动物模型中的光遗传学方法来探究PF在AIMs发展中的作用。
对14只大鼠进行立体定向手术，向其PF外侧三分之一注射携带视紫红质通道蛋白的腺相关病毒（AAV2-hSyn-ChR2-mCherry）。在注射病毒14天后，分别在有光刺激和无光刺激的情况下进行行为测试。使用AIM评分来检测大鼠的AIMs。行为测试后，小心取出大鼠大脑，并用荧光显微镜检查切片以确认PF的转染情况。
14只大鼠中，7只表现出明显的不自主异常运动。与基线相比，刺激后AIM评分显著增加。在出现AIMs的大鼠中，PF中mCherry表达明显，而未出现AIMs的大鼠则缺乏mCherry表达。
通过光遗传学方法刺激PF可可逆地诱导AIMs。
